Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 42922..43499 Replicon plasmid pKp_Goe_414-3
Accession NZ_CP018340
Organism Klebsiella pneumoniae isolate Kp_Goe_154414

Toxin (Protein)


Gene name mazF Uniprot ID A0A7G3NNX6
Locus tag BB788_RS29360 Protein ID WP_059065587.1
Coordinates 43167..43499 (+) Length 111 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A7G3NP82
Locus tag BB788_RS29355 Protein ID WP_032454911.1
Coordinates 42922..43167 (+) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BB788_RS29305 37937..38581 + 645 WP_064291676.1 hypothetical protein -
BB788_RS29310 38637..39287 + 651 WP_014343480.1 DUF2726 domain-containing protein -
BB788_RS29315 39284..39592 + 309 WP_004152383.1 hypothetical protein -
BB788_RS29320 39768..40247 + 480 WP_014343478.1 phospholipase D family protein -
BB788_RS29325 40365..40634 + 270 WP_013609506.1 replication regulatory protein RepA -
BB788_RS29330 40866..40943 + 78 WP_004171457.1 RepA leader peptide Tap -
BB788_RS29335 40936..41793 + 858 WP_013023768.1 incFII family plasmid replication initiator RepA -
BB788_RS29355 42922..43167 + 246 WP_032454911.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
BB788_RS29360 43167..43499 + 333 WP_059065587.1 endoribonuclease MazF Toxin
BB788_RS29365 43759..44082 + 324 WP_073558199.1 hypothetical protein -
BB788_RS29370 44265..44519 - 255 WP_073558200.1 hypothetical protein -
BB788_RS29375 44595..44852 - 258 WP_073558201.1 hypothetical protein -
BB788_RS29380 44901..45104 - 204 WP_004152106.1 hemolysin expression modulator Hha -
BB788_RS29385 45138..45506 - 369 WP_073558202.1 hypothetical protein -
BB788_RS29390 45550..46044 - 495 WP_073558203.1 DNA-binding protein -
BB788_RS29395 46075..46650 - 576 WP_073558204.1 hypothetical protein -
BB788_RS29400 46638..46907 - 270 WP_073558205.1 hypothetical protein -
BB788_RS29415 47503..47721 + 219 WP_001568025.1 type II toxin-antitoxin system antitoxin CcdA -
BB788_RS29420 47723..48028 + 306 WP_004197633.1 type II toxin-antitoxin system toxin CcdB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..81939 81939


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(9-107)

Antitoxin

SpoVT-AbrB domain

(7-50)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11991.87 Da        Isoelectric Point: 7.7494

>T69771 WP_059065587.1 NZ_CP018340:43167-43499 [Klebsiella pneumoniae]
MVSRFVPDAGDLIWINFDPVEGHEQGGHRPAVVLSPFAYNNKTGLLLCVPCTTKVKGYPFEVELPGERDGVALADQITCV
DWRARKVEKKGKVATGELSEIRAKAKALIG

Download         Length: 333 bp

>T69771 NZ_CP018340:43167-43499 [Klebsiella pneumoniae]
ATGGTATCTCGTTTTGTGCCGGATGCTGGCGACCTTATCTGGATAAATTTTGATCCCGTGGAAGGACATGAGCAAGGAGG
GCATCGCCCCGCAGTCGTGCTGAGTCCGTTTGCATACAACAACAAAACAGGTTTGTTGCTATGCGTACCTTGCACAACAA
AAGTAAAAGGCTATCCCTTTGAAGTTGAATTGCCAGGAGAACGCGATGGTGTAGCTCTTGCAGATCAAATAACGTGTGTA
GACTGGCGAGCCAGAAAAGTAGAAAAGAAGGGAAAGGTTGCCACCGGAGAATTGTCAGAAATCAGAGCTAAAGCAAAAGC
CTTAATCGGTTGA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 8881.21 Da        Isoelectric Point: 5.7996

>AT69771 WP_032454911.1 NZ_CP018340:42922-43167 [Klebsiella pneumoniae]
MSQATFKKWGNQAGIRFPAAVMKAAALNVDDTVDIEVQEGRIVLIPTKEKTYSLDALLSGITEENMHNKADFGKPTGKEM
L

Download         Length: 246 bp

>AT69771 NZ_CP018340:42922-43167 [Klebsiella pneumoniae]
ATGTCACAGGCAACGTTCAAGAAATGGGGTAACCAGGCAGGAATTCGTTTTCCGGCAGCGGTGATGAAAGCAGCAGCGCT
TAACGTAGATGATACGGTTGATATTGAGGTACAGGAAGGGCGGATTGTACTCATACCAACGAAAGAAAAGACCTATTCAC
TCGATGCCTTATTATCCGGCATCACCGAAGAAAACATGCATAACAAAGCTGACTTCGGCAAACCGACAGGGAAGGAAATG
CTCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7G3NNX6

Antitoxin

Source ID Structure
AlphaFold DB A0A7G3NP82

References