Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 89234..89970 Replicon plasmid pKp_Goe_414-2
Accession NZ_CP018338
Organism Klebsiella pneumoniae isolate Kp_Goe_154414

Toxin (Protein)


Gene name tacT Uniprot ID A0A2J5Q928
Locus tag BB788_RS27270 Protein ID WP_004098919.1
Coordinates 89488..89970 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID A0A7D3T1D0
Locus tag BB788_RS27265 Protein ID WP_004213599.1
Coordinates 89234..89500 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BB788_RS27245 86748..87752 + 1005 WP_073558142.1 IS110-like element IS4321 family transposase -
BB788_RS27250 88038..88532 + 495 WP_004213594.1 hypothetical protein -
BB788_RS27255 88593..88796 + 204 WP_004213596.1 hemolysin expression modulator Hha -
BB788_RS27260 88810..89040 + 231 WP_004213598.1 hypothetical protein -
BB788_RS27265 89234..89500 + 267 WP_004213599.1 DUF1778 domain-containing protein Antitoxin
BB788_RS27270 89488..89970 + 483 WP_004098919.1 GNAT family N-acetyltransferase Toxin
BB788_RS31365 90391..91618 + 1228 Protein_100 IS3 family transposase -
BB788_RS27290 91614..92009 - 396 Protein_101 DDE-type integrase/transposase/recombinase -
BB788_RS27300 92184..93206 - 1023 WP_004214536.1 porphobilinogen synthase -
BB788_RS27305 93270..94616 - 1347 WP_048333011.1 dihydroorotase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - rmpA / iutA / iucD / iucC / iucB / iucA / rmpA / rmpA / iroN 1..202175 202175
- inside IScluster/Tn - rmpA / rmpA / iroN 59870..91618 31748


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(66-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17335.06 Da        Isoelectric Point: 10.0704

>T69769 WP_004098919.1 NZ_CP018338:89488-89970 [Klebsiella pneumoniae]
VGRITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKKGTKKVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T69769 NZ_CP018338:89488-89970 [Klebsiella pneumoniae]
GTGGGACGTATAACCGCGCCCGAGCCGTTATCCAGCGCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCACTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGAAGGGTACGA
AGAAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCTACAGGCAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGTGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGCGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10151.58 Da        Isoelectric Point: 4.5141

>AT69769 WP_004213599.1 NZ_CP018338:89234-89500 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRVLIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDDSAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT69769 NZ_CP018338:89234-89500 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGTACTCATCGATGCCGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGATTCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J5Q928

Antitoxin

Source ID Structure
AlphaFold DB A0A7D3T1D0

References