Detailed information of TA system TA069768

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 32844..33514
• Replicon: plasmid pKp_Goe_414-2
• Accession: NZ_CP018338
• Organism: Klebsiella pneumoniae isolate Kp_Goe_154414

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: BB788_RS26910
• Protein ID: WP_004213072.1
• Coordinates: 33071..33514 (+)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: BB788_RS26905
• Protein ID: WP_004213073.1
• Coordinates: 32844..33074 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BB788_RS26875	29067..29966	-	900	WP_004225022.1	nucleotide-binding protein	-
BB788_RS26880	29956..30246	-	291	WP_004213078.1	hypothetical protein	-
BB788_RS26885	30598..30804	+	207	WP_004213077.1	hypothetical protein	-
BB788_RS26890	30794..31087	-	294	WP_004213076.1	hypothetical protein	-
BB788_RS26895	31103..32236	-	1134	WP_004213075.1	DUF3800 domain-containing protein	-
BB788_RS26905	32844..33074	+	231	WP_004213073.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
BB788_RS26910	33071..33514	+	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
BB788_RS26915	33528..33944	-	417	Protein_36	restriction endonuclease	-
BB788_RS26920	33948..34241	-	294	Protein_37	transposase	-
BB788_RS32680	34658..35292	+	635	Protein_38	response regulator	-
BB788_RS26935	35807..36213	-	407	Protein_39	GAF domain-containing protein	-
BB788_RS26945	36304..37224	-	921	WP_004213934.1	DUF1471 domain-containing protein	-
BB788_RS26950	37273..37764	-	492	WP_011251327.1	DM13 domain-containing protein	-
BB788_RS26955	37827..38102	-	276	WP_004213932.1	carboxymuconolactone decarboxylase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	rmpA / iutA / iucD / iucC / iucB / iucA / rmpA / rmpA / iroN	1..202175	202175

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T69768 WP_004213072.1 NZ_CP018338:33071-33514 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T69768 NZ_CP018338:33071-33514 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAACAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
TGGGCACGCTATCGCGGCCGGCGCCGTCCTAGTGACGAACAATGTGAGAGAGTTTGAGCGGGTGCCGGGTCTGGTGCTGG
AAGACTGGGTGAAGAAAGCCGCTCTCTGCAGGGTAATTTCTTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT69768 WP_004213073.1 NZ_CP018338:32844-33074 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT69768 NZ_CP018338:32844-33074 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGCTCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620