Detailed information of TA system TA069749

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 2582..3204
• Replicon: plasmid pA1296_2
• Accession: NZ_CP018334
• Organism: Acinetobacter baumannii strain A1296 isolate A1296

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A9E9L1H0
• Locus tag: BS103_RS18355
• Protein ID: WP_075041255.1
• Coordinates: 2809..3204 (+)
• Length: 132 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A3A8E7V1
• Locus tag: BS103_RS18350
• Protein ID: WP_012268402.1
• Coordinates: 2582..2812 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BS103_RS18335	79..1266	-	1188	WP_004856455.1	tetracycline efflux MFS transporter Tet(39)	-
BS103_RS18340	1332..1973	+	642	WP_004787586.1	TetR/AcrR family transcriptional regulator C-terminal domain-containing protein	-
BS103_RS18350	2582..2812	+	231	WP_012268402.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
BS103_RS18355	2809..3204	+	396	WP_075041255.1	type II toxin-antitoxin system VapC family toxin	Toxin
BS103_RS18360	3359..3571	+	213	WP_080632801.1	helix-turn-helix domain-containing protein	-
BS103_RS18365	3585..4745	+	1161	WP_097355496.1	hypothetical protein	-
BS103_RS18370	4825..6726	+	1902	WP_097355497.1	N-6 DNA methylase	-
BS103_RS18375	6763..6984	-	222	WP_097355498.1	hypothetical protein	-
BS103_RS18380	6981..7337	-	357	WP_097355499.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	tet(39)	-	1..11586	11586

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-124)

Antitoxin

• SpoVT-AbrB domain (7-37)

Sequences

Toxin

Protein

• Length: 132 a.a.
• Molecular weight: 14919.24 Da
• Isoelectric Point: 6.2253

>T69749 WP_075041255.1 NZ_CP018334:2809-3204 [Acinetobacter baumannii]
MIYLLDTNICIYVINNKPQHVFERFKQYQLGQLAISSITASELAFGVEKSGSERNKQALNKFLSPLEILPYDEQAIWHYA
KLRQDLQSIGKPIGSLDMLIAAHALALDVVLVTNNTKEFERIDGLKLENWV

Nucleotide

• Length: 396 bp

>T69749 NZ_CP018334:2809-3204 [Acinetobacter baumannii]
ATGATTTACCTCTTAGACACAAATATTTGTATCTATGTCATCAACAACAAGCCTCAACACGTTTTTGAACGCTTTAAGCA
ATATCAACTCGGCCAGCTTGCGATTTCAAGTATCACGGCATCTGAGCTGGCTTTCGGTGTAGAGAAGTCTGGATCTGAAC
GCAATAAACAGGCATTAAACAAGTTTTTGTCACCTTTAGAGATTCTTCCTTACGATGAACAAGCAATTTGGCATTACGCC
AAATTACGCCAAGATCTTCAATCCATAGGCAAACCGATTGGCAGCTTAGATATGCTGATCGCTGCTCATGCTTTGGCATT
GGATGTTGTATTAGTAACGAACAATACAAAAGAATTTGAACGGATTGATGGTCTAAAGCTAGAGAATTGGGTCTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8765.04 Da
• Isoelectric Point: 4.7708

>AT69749 WP_012268402.1 NZ_CP018334:2582-2812 [Acinetobacter baumannii]
MEIAKVFQTGRSQAVRLPKAFRFNGTEVAIKSFGRGVLLMPIDNPWDVMLEAINEFEVGFQLERADQGEQFREEFK

Nucleotide

• Length: 231 bp

>AT69749 NZ_CP018334:2582-2812 [Acinetobacter baumannii]
ATGGAAATTGCCAAAGTATTTCAAACTGGTCGCAGTCAAGCGGTTCGCTTGCCTAAGGCCTTTCGTTTCAATGGCACTGA
AGTGGCCATCAAAAGCTTCGGACGTGGCGTTTTGTTGATGCCAATCGATAACCCTTGGGATGTCATGTTAGAAGCCATCA
ATGAGTTTGAGGTTGGATTCCAGTTGGAGCGAGCTGATCAAGGCGAACAGTTCCGTGAGGAGTTCAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A3A8E7V1