Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 2843676..2844294 Replicon chromosome
Accession NZ_CP018323
Organism Escherichia coli strain KSC9

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag BS635_RS14600 Protein ID WP_001291435.1
Coordinates 2843676..2843894 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag BS635_RS14605 Protein ID WP_000344800.1
Coordinates 2843920..2844294 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BS635_RS14570 2838850..2839470 + 621 WP_001267012.1 recombinase family protein -
BS635_RS14575 2839688..2839915 + 228 WP_024183738.1 DUF2188 domain-containing protein -
BS635_RS14580 2840259..2840612 + 354 WP_000878141.1 DUF1428 family protein -
BS635_RS14585 2840654..2842204 - 1551 WP_001299455.1 cyclic-guanylate-specific phosphodiesterase PdeB -
BS635_RS14590 2842368..2842838 - 471 WP_000136192.1 YlaC family protein -
BS635_RS14595 2842954..2843505 - 552 WP_000102564.1 maltose O-acetyltransferase -
BS635_RS14600 2843676..2843894 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
BS635_RS14605 2843920..2844294 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
BS635_RS14610 2844840..2847989 - 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
BS635_RS14615 2848012..2849205 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T69733 WP_001291435.1 NZ_CP018323:c2843894-2843676 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T69733 NZ_CP018323:c2843894-2843676 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTATACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT69733 WP_000344800.1 NZ_CP018323:c2844294-2843920 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT69733 NZ_CP018323:c2844294-2843920 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References