Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 1212602..1213233 Replicon chromosome
Accession NZ_CP018323
Organism Escherichia coli strain KSC9

Toxin (Protein)


Gene name hicA Uniprot ID F4NNK6
Locus tag BS635_RS06415 Protein ID WP_001259388.1
Coordinates 1212958..1213233 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag BS635_RS06410 Protein ID WP_000593555.1
Coordinates 1212602..1212961 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BS635_RS06385 1208734..1209678 + 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -
BS635_RS06390 1209675..1210508 + 834 WP_087530351.1 nickel ABC transporter permease subunit NikC -
BS635_RS06395 1210508..1211272 + 765 WP_001136236.1 nickel import ATP-binding protein NikD -
BS635_RS06400 1211269..1212075 + 807 WP_000173666.1 nickel import ATP-binding protein NikE -
BS635_RS06405 1212081..1212482 + 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
BS635_RS06410 1212602..1212961 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
BS635_RS06415 1212958..1213233 - 276 WP_001259388.1 type II toxin-antitoxin system HicA family toxin Toxin
BS635_RS06420 1213306..1214430 - 1125 WP_001314210.1 ABC transporter permease -
BS635_RS06425 1214430..1217165 - 2736 WP_000149125.1 ribosome-associated ATPase/putative transporter RbbA -
BS635_RS06430 1217162..1218229 - 1068 WP_000361485.1 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10151.89 Da        Isoelectric Point: 10.7076

>T69726 WP_001259388.1 NZ_CP018323:c1213233-1212958 [Escherichia coli]
MRTKVQALRKKQKNTLDQIVKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T69726 NZ_CP018323:c1213233-1212958 [Escherichia coli]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATAGTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGCAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT69726 WP_000593555.1 NZ_CP018323:c1212961-1212602 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT69726 NZ_CP018323:c1212961-1212602 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCAGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A0FFA2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References