Detailed information of TA system TA069722

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 186421..187046
• Replicon: chromosome
• Accession: NZ_CP018323
• Organism: Escherichia coli strain KSC9

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: BS635_RS01020
• Protein ID: WP_000911330.1
• Coordinates: 186421..186819 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: BS635_RS01025
• Protein ID: WP_000450524.1
• Coordinates: 186819..187046 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BS635_RS01000	182328..182528	+	201	WP_000383836.1	YpfN family protein	-
BS635_RS01005	182609..183307	-	699	WP_000679812.1	esterase	-
BS635_RS01010	183381..185396	-	2016	WP_000829292.1	tRNA cytosine(34) acetyltransferase TmcA	-
BS635_RS01015	185411..186274	-	864	WP_001267498.1	neutral zinc metallopeptidase	-
BS635_RS01020	186421..186819	-	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
BS635_RS01025	186819..187046	-	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
BS635_RS01030	187200..187913	-	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
BS635_RS01035	188126..189160	-	1035	WP_001330579.1	outer membrane protein assembly factor BamC	-
BS635_RS01040	189177..190055	-	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
BS635_RS01045	190201..190773	+	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
BS635_RS01050	190773..191243	+	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T69722 WP_000911330.1 NZ_CP018323:c186819-186421 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T69722 NZ_CP018323:c186819-186421 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT69722 WP_000450524.1 NZ_CP018323:c187046-186819 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT69722 NZ_CP018323:c187046-186819 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33