Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PumAB/COG3657-DUF971
Location 43680..44275 Replicon plasmid unnamed
Accession NZ_CP018320
Organism Pseudomonas frederiksbergensis strain AS1

Toxin (Protein)


Gene name PumA Uniprot ID C3KFR0
Locus tag PFAS1_RS28910 Protein ID WP_011117437.1
Coordinates 43973..44275 (-) Length 101 a.a.

Antitoxin (Protein)


Gene name PumB Uniprot ID C3KFQ9
Locus tag PFAS1_RS28905 Protein ID WP_011117436.1
Coordinates 43680..43976 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PFAS1_RS28870 39312..40214 + 903 WP_011117383.1 HTH-type transcriptional activator NahR -
PFAS1_RS28875 40274..40653 + 380 Protein_47 IS30 family transposase -
PFAS1_RS28885 40824..41771 + 948 Protein_48 tyrosine-type recombinase/integrase -
PFAS1_RS28895 42142..42657 - 516 WP_173495103.1 hypothetical protein -
PFAS1_RS28900 42671..43095 - 425 Protein_50 S24 family peptidase -
PFAS1_RS28905 43680..43976 - 297 WP_011117436.1 putative addiction module antidote protein Antitoxin
PFAS1_RS28910 43973..44275 - 303 WP_011117437.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
PFAS1_RS28915 44485..44889 + 405 WP_011117438.1 hypothetical protein -
PFAS1_RS28920 44940..45424 + 485 Protein_54 single-stranded DNA-binding protein -
PFAS1_RS28925 45619..46446 + 828 WP_011117440.1 DUF945 domain-containing protein -
PFAS1_RS28930 46501..46923 + 423 WP_011117441.1 hypothetical protein -
PFAS1_RS28935 46947..47276 + 330 WP_011117442.1 hypothetical protein -
PFAS1_RS28940 47269..47541 + 273 WP_019694539.1 hypothetical protein -
PFAS1_RS28945 47808..48071 + 264 WP_019694540.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..81841 81841


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(41-91)

Antitoxin

Uncharacterized protein HI1420

(6-89)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11175.89 Da        Isoelectric Point: 10.1783

>T69720 WP_011117437.1 NZ_CP018320:c44275-43973 [Pseudomonas frederiksbergensis]
MKTIIQTATYMTWERKLRDKKAKLIIAARVLRVAHGLVGDVQPVGQGVSELRIHHGPGYRVYFQQRGDQLVLLLCGGDKS
SQARDIETAKTLASQWSADE

Download         Length: 303 bp

>T69720 NZ_CP018320:c44275-43973 [Pseudomonas frederiksbergensis]
ATGAAAACGATTATCCAAACCGCCACTTACATGACTTGGGAACGCAAACTGCGTGACAAGAAGGCCAAACTGATTATCGC
GGCGCGGGTGCTGCGGGTAGCCCACGGGTTGGTCGGTGACGTGCAGCCAGTAGGTCAAGGCGTCAGTGAGTTGCGAATTC
ATCATGGCCCCGGTTACCGGGTGTATTTCCAGCAGCGCGGCGATCAGCTGGTCCTATTGCTCTGCGGTGGGGATAAAAGC
AGTCAGGCGCGGGATATAGAAACCGCTAAAACTCTGGCAAGCCAGTGGAGTGCAGACGAATGA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 10475.98 Da        Isoelectric Point: 4.5515

>AT69720 WP_011117436.1 NZ_CP018320:c43976-43680 [Pseudomonas frederiksbergensis]
MKEPIYEYDPAELLTDAESIAIFMADAFESGDASFIAQALSVVARAKGMTSIAEEAGVTRAHLYNLKNGNPTLKTMIGVM
RAVGLDMTAKQHVDLANG

Download         Length: 297 bp

>AT69720 NZ_CP018320:c43976-43680 [Pseudomonas frederiksbergensis]
ATGAAAGAACCAATTTACGAATACGATCCAGCGGAATTACTCACTGACGCGGAATCTATCGCCATCTTTATGGCTGATGC
TTTCGAATCGGGGGATGCCTCCTTTATCGCGCAAGCGCTGAGCGTGGTAGCTCGCGCCAAAGGCATGACCTCAATTGCAG
AAGAGGCAGGCGTCACCCGCGCACACCTCTACAATCTGAAAAACGGCAATCCGACATTGAAAACCATGATCGGCGTGATG
CGTGCGGTAGGCCTGGACATGACAGCAAAACAGCACGTCGACCTAGCCAACGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1P8F4M5

Antitoxin

Source ID Structure
AlphaFold DB A0A1P8F4B5

References