Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 14580..15223 Replicon plasmid pKp_Goe_579-4
Accession NZ_CP018316
Organism Klebsiella pneumoniae strain Kp_Goe_822579

Toxin (Protein)


Gene name vagD Uniprot ID A0A7Z7T700
Locus tag BB744_RS29875 Protein ID WP_040155119.1
Coordinates 14580..14996 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag BB744_RS29880 Protein ID WP_001261276.1
Coordinates 14993..15223 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BB744_RS29850 10025..10722 - 698 Protein_13 IS1 family transposase -
BB744_RS29860 10779..11732 - 954 Protein_14 colicin V synthesis protein -
BB744_RS29865 11722..12999 - 1278 Protein_15 HlyD family secretion protein -
BB744_RS29870 13661..14539 + 879 WP_009309916.1 restriction endonuclease -
BB744_RS29875 14580..14996 - 417 WP_040155119.1 type II toxin-antitoxin system VapC family toxin Toxin
BB744_RS29880 14993..15223 - 231 WP_001261276.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
BB744_RS29890 15797..16147 + 351 WP_000493378.1 hypothetical protein -
BB744_RS29895 16198..16941 + 744 WP_000129823.1 hypothetical protein -
BB744_RS29900 16938..17714 + 777 WP_000015958.1 site-specific integrase -
BB744_RS29905 17772..18029 - 258 WP_000764642.1 hypothetical protein -
BB744_RS33015 18158..18262 - 105 WP_032409716.1 hypothetical protein -
BB744_RS29920 18797..19663 + 867 WP_004118283.1 replication initiation protein -
BB744_RS29925 19840..20109 - 270 WP_000339857.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid sul1 / qacE / dfrA1 / tet(A) / aph(3'')-Ib / aph(6)-Id / blaCTX-M-15 / blaTEM-1B / qnrS1 - 1..61012 61012


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15147.60 Da        Isoelectric Point: 7.8915

>T69712 WP_040155119.1 NZ_CP018316:c14996-14580 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPESVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIHLVDAFCARLDAVLPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNVREFERVPGLVLEDWVK

Download         Length: 417 bp

>T69712 NZ_CP018316:c14996-14580 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGAATCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGTGACCGCATTGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCATCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCGTCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCGGCGGGCGCCATCCTGGTGACGAACAATGTGAGAGAGTTTGAGCGGGTGCCGGGTCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT69712 WP_001261276.1 NZ_CP018316:c15223-14993 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT69712 NZ_CP018316:c15223-14993 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGATGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7Z7T700

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References