Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 5058916..5059691 Replicon chromosome
Accession NZ_CP018307
Organism Klebsiella variicola strain X39

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A2N4Z6P0
Locus tag BC497_RS25095 Protein ID WP_032740995.1
Coordinates 5059206..5059691 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag BC497_RS25090 Protein ID WP_004150912.1
Coordinates 5058916..5059209 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BC497_RS25070 5054172..5054774 - 603 WP_012540553.1 short chain dehydrogenase -
BC497_RS25075 5054872..5055783 + 912 WP_023298220.1 LysR family transcriptional regulator -
BC497_RS25080 5055784..5056932 - 1149 WP_008806488.1 PLP-dependent aspartate aminotransferase family protein -
BC497_RS25085 5056944..5058320 - 1377 WP_016161835.1 cystathionine beta-synthase -
BC497_RS25090 5058916..5059209 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
BC497_RS25095 5059206..5059691 + 486 WP_032740995.1 GNAT family N-acetyltransferase Toxin
BC497_RS25100 5060395..5060988 + 594 WP_004188553.1 hypothetical protein -
BC497_RS25105 5061085..5061301 + 217 Protein_4767 transposase -
BC497_RS25120 5061816..5062529 - 714 WP_008806478.1 DUF554 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 5061085..5061237 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17618.64 Da        Isoelectric Point: 8.5178

>T69704 WP_032740995.1 NZ_CP018307:5059206-5059691 [Klebsiella variicola]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPDPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVH
A

Download         Length: 486 bp

>T69704 NZ_CP018307:5059206-5059691 [Klebsiella variicola]
ATGATCTCGGCACCAGAACCGCTTCATGCCGGGCATATTCTTACCCCGTTTTGCTGCGGTATAGATTCCATGGATCACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTTACTGGCGCATCCCGCACCTTTGTCTGCTGCGACGACGCGAAGGTGATGG
CTTACTACTCACTGGCTTCCAGCGCTGTGACGACAAATACTGCGCCGGGTCGTTTTCGCCGCAATATGCCTGACCCGATC
CCGGTTGTGGTACTGGGTCGTCTGGCGGTGGATAAATCACTACATGGGAAAGGTGTCGGGCGGGCCCTGGTACGTGATGC
CGGGCTGCGTGTGATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCACGGG
ATTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGAGGGATTTGGTTCAT
GCCTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT69704 WP_004150912.1 NZ_CP018307:5058916-5059209 [Klebsiella variicola]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT69704 NZ_CP018307:5058916-5059209 [Klebsiella variicola]
ATGCCAGCCGCAAACAGCATGTCCATAAAACGCGAAACGTTAAATCTGCGCATTAAACCTGCCGAACGCGATCTTATTGA
TCGGGCGGCGAAAGCCAGAGGGAAAAACCGCACCGACTTTGTGCTGGAGGCCGCCCGCGCTGCCGCTGAGGAAGCGCTGA
TTGAGCAACGCATCATTATGGCCGATCCGCAGGCTTATCAGGAATTTCTCGCTCGTCTGGATCAGGCTCCTGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCCTGCGCCGTGGGAACAGGAAGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2N4Z6P0

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References