Detailed information of TA system TA069702

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 4642629..4643215
• Replicon: chromosome
• Accession: NZ_CP018307
• Organism: Klebsiella variicola strain X39

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: BC497_RS22855
• Protein ID: WP_087653349.1
• Coordinates: 4642847..4643215 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: A0A1F2M0C2
• Locus tag: BC497_RS22850
• Protein ID: WP_032731361.1
• Coordinates: 4642629..4642850 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BC497_RS22830	4638786..4639712	+	927	WP_012540389.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
BC497_RS22835	4639709..4640986	+	1278	WP_008806971.1	branched chain amino acid ABC transporter permease LivM	-
BC497_RS22840	4640983..4641750	+	768	WP_008806972.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-
BC497_RS22845	4641752..4642465	+	714	WP_004145133.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF	-
BC497_RS22850	4642629..4642850	+	222	WP_032731361.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
BC497_RS22855	4642847..4643215	+	369	WP_087653349.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
BC497_RS22860	4643507..4644823	+	1317	WP_087653350.1	sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB	-
BC497_RS22865	4644930..4645817	+	888	WP_012967120.1	sn-glycerol-3-phosphate ABC transporter permease UgpA	-
BC497_RS22870	4645814..4646659	+	846	WP_008806976.1	sn-glycerol-3-phosphate ABC transporter permease UgpE	-
BC497_RS22875	4646661..4647731	+	1071	WP_008806977.1	sn-glycerol-3-phosphate import ATP-binding protein UgpC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4639709..4648468	8759

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (13-86)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-50)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13499.88 Da
• Isoelectric Point: 7.2780

>T69702 WP_087653349.1 NZ_CP018307:4642847-4643215 [Klebsiella variicola]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISCGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Nucleotide

• Length: 369 bp

>T69702 NZ_CP018307:4642847-4643215 [Klebsiella variicola]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTTGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCGGC
AGCGGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGCGGATGA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8165.18 Da
• Isoelectric Point: 4.7769

>AT69702 WP_032731361.1 NZ_CP018307:4642629-4642850 [Klebsiella variicola]
MRTVNYSEARQNLADVLESAVTGIPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Nucleotide

• Length: 222 bp

>AT69702 NZ_CP018307:4642629-4642850 [Klebsiella variicola]
ATGAGAACGGTTAACTATAGCGAAGCCCGACAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTATACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCGGTCATCATTAGTGCAGAAGAATTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATTATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A1F2M0C2