Detailed information of TA system TA069698

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yfjZ-ypjF/CbtA-CbeA
• Location: 3198733..3199439
• Replicon: chromosome
• Accession: NZ_CP018307
• Organism: Klebsiella variicola strain X39

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: -
• Locus tag: BC497_RS15860
• Protein ID: WP_087653325.1
• Coordinates: 3199071..3199439 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: A0A485WAM6
• Locus tag: BC497_RS15855
• Protein ID: WP_023302278.1
• Coordinates: 3198733..3199050 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BC497_RS15810	3193874..3194698	+	825	WP_087653324.1	DUF945 domain-containing protein	-
BC497_RS15815	3194907..3195617	+	711	WP_023302272.1	DeoR family transcriptional regulator	-
BC497_RS15820	3195643..3196179	+	537	WP_023302273.1	DUF4339 domain-containing protein	-
BC497_RS15825	3196221..3196658	+	438	WP_023301392.1	hypothetical protein	-
BC497_RS15830	3196725..3197135	+	411	WP_023302274.1	hypothetical protein	-
BC497_RS15835	3197213..3197449	+	237	WP_032410024.1	DUF905 domain-containing protein	-
BC497_RS15840	3197536..3197994	+	459	WP_023302275.1	antirestriction protein	-
BC497_RS15845	3198003..3198485	+	483	WP_023302276.1	RadC family protein	-
BC497_RS15850	3198494..3198715	+	222	WP_023302277.1	DUF987 domain-containing protein	-
BC497_RS15855	3198733..3199050	+	318	WP_023302278.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
BC497_RS15860	3199071..3199439	+	369	WP_087653325.1	TA system toxin CbtA family protein	Toxin
BC497_RS15870	3199887..3202531	-	2645	Protein_3058	helix-turn-helix transcriptional regulator	-
BC497_RS15875	3202904..3203863	+	960	WP_087653328.1	thiamine pyrophosphate-dependent dehydrogenase E1 component subunit alpha	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-111)

Antitoxin

• Antitoxin CbeA (4-104)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13669.85 Da
• Isoelectric Point: 7.2897

>T69698 WP_087653325.1 NZ_CP018307:3199071-3199439 [Klebsiella variicola]
MKNLPATISQAAKPCLSPVAVWQMLLTHLLEQHYGLMLSDTPFSDEAVIQEHIDAGITLANAVNFLVEKYELVRIDRCGF
SSQVQAPYLTATDILHARKACGLMSRYSYREVSNIVLSRSRM

Nucleotide

• Length: 369 bp

>T69698 NZ_CP018307:3199071-3199439 [Klebsiella variicola]
ATGAAAAATCTACCTGCTACAATTTCGCAGGCGGCGAAGCCCTGTCTGTCGCCCGTTGCAGTCTGGCAAATGTTACTTAC
TCATCTGCTTGAGCAGCACTATGGCCTAATGCTAAGCGACACACCTTTTAGTGATGAAGCCGTTATACAGGAACATATTG
ATGCCGGTATCACTCTGGCTAATGCTGTTAACTTTCTGGTGGAGAAGTATGAGTTAGTCCGTATCGATCGCTGTGGATTT
TCCTCACAGGTACAAGCACCATATTTGACTGCGACAGACATTCTTCATGCCAGGAAAGCGTGCGGGCTGATGAGCCGGTA
TTCTTACAGGGAGGTAAGTAATATTGTTCTTAGCCGATCACGGATGTGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11860.67 Da
• Isoelectric Point: 6.4629

>AT69698 WP_023302278.1 NZ_CP018307:3198733-3199050 [Klebsiella variicola]
MSNPTWGLLRDITPCLGARLVQEGNRLYYLVDRASITGKFSDAEILKLDVVFPHFISQMESMLTIGEMNPRHAHCVTLYH
NGFTCEADTLGSCGYVYIAIYLTQR

Nucleotide

• Length: 318 bp

>AT69698 NZ_CP018307:3198733-3199050 [Klebsiella variicola]
ATGAGCAACCCCACATGGGGCCTGCTGCGGGATATCACGCCGTGCCTAGGAGCACGTCTGGTGCAGGAGGGCAACCGGCT
GTACTATCTGGTTGACCGAGCAAGTATCACCGGTAAGTTTAGTGATGCCGAAATCCTGAAGTTGGATGTCGTATTTCCAC
ATTTTATCAGTCAGATGGAATCGATGCTGACCATTGGTGAAATGAATCCCCGCCATGCCCACTGTGTCACCCTGTACCAC
AACGGTTTCACCTGCGAAGCCGATACCCTTGGTAGTTGCGGCTACGTATACATCGCCATTTATCTCACTCAACGCTAA

Structures

Antitoxin

• AlphaFold DB: A0A485WAM6