Detailed information of TA system TA069686

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 637839..638429
• Replicon: chromosome
• Accession: NZ_CP018307
• Organism: Klebsiella variicola strain X39

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: BC497_RS03015
• Protein ID: WP_023322934.1
• Coordinates: 638097..638429 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: A0A1F2LZQ3
• Locus tag: BC497_RS03010
• Protein ID: WP_012541132.1
• Coordinates: 637839..638096 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BC497_RS02985	633449..634024	+	576	WP_032733307.1	hypothetical protein	-
BC497_RS02990	634202..635038	+	837	WP_008804155.1	alpha/beta hydrolase	-
BC497_RS02995	635244..636215	+	972	WP_048293502.1	sensor domain-containing diguanylate cyclase	-
BC497_RS03000	636212..637312	-	1101	WP_087653750.1	phosphotransferase	-
BC497_RS03010	637839..638096	+	258	WP_012541132.1	antitoxin	Antitoxin
BC497_RS03015	638097..638429	+	333	WP_023322934.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
BC497_RS03020	638493..638595	+	103	Protein_577	DNA primase	-
BC497_RS03025	638833..639405	-	573	WP_032738874.1	TetR/AcrR family transcriptional regulator	-
BC497_RS03030	639600..640310	-	711	WP_023322932.1	SDR family oxidoreductase	-
BC497_RS03035	640297..640617	-	321	WP_023322931.1	Dabb family protein	-
BC497_RS03040	640610..641482	-	873	WP_087653749.1	sugar transporter	-
BC497_RS03045	641493..643214	-	1722	WP_023322929.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-108)

Antitoxin

• Antitoxin PemI-like (4-84)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11768.58 Da
• Isoelectric Point: 9.2926

>T69686 WP_023322934.1 NZ_CP018307:638097-638429 [Klebsiella variicola]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPVTSGGNFARTAGFTVSLEEAGTKTTGVIRCDQPGTI
DMAARNGKRLERIPDAVVNEVLARLDAILS

Nucleotide

• Length: 333 bp

>T69686 NZ_CP018307:638097-638429 [Klebsiella variicola]
ATGGACAGAGGGGAAATCTGGCTTGTTTCACTGGATCCGATTGCTGGCCACGAGCAAAGTGGAAAACGTCCGGTCCTCAT
TGTATCGAAGGCATCGTTTAACAAGCTGACCCGGCTACCGGTCGTGGTTCCCGTCACCAGCGGCGGAAACTTTGCACGTA
CAGCAGGCTTCACGGTTTCACTGGAAGAGGCGGGGACCAAAACAACAGGCGTTATTCGCTGCGACCAGCCTGGAACTATC
GATATGGCGGCCCGGAACGGCAAGCGTCTGGAACGCATACCTGACGCCGTTGTCAATGAAGTGCTTGCCAGGCTGGATGC
GATTCTGAGTTAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9420.91 Da
• Isoelectric Point: 4.4915

>AT69686 WP_012541132.1 NZ_CP018307:637839-638096 [Klebsiella variicola]
MYKTRLKKVGGSIMLAVPPAVLKALELSTDSEVGMTIDNGCLIIEPQKRPHYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Nucleotide

• Length: 258 bp

>AT69686 NZ_CP018307:637839-638096 [Klebsiella variicola]
ATGTACAAAACTCGCCTGAAAAAGGTTGGCGGATCCATCATGCTGGCGGTTCCTCCCGCCGTGCTGAAAGCGCTGGAGCT
GTCGACAGACAGCGAAGTGGGTATGACTATAGATAATGGCTGCCTGATTATTGAACCGCAGAAACGGCCCCATTATTCGC
TTGAGGAACTGTTGGCGCAGTGCGATCCGCACGCCGAAATGAGCGAAGAGGATCGGGAATGGATTGATGCGCCTGCGGTG
GGCAAGGAGATTCTGTAA

Structures

Antitoxin

• AlphaFold DB: A0A1F2LZQ3