Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 1844794..1845419 Replicon chromosome
Accession NZ_CP018306
Organism Klebsiella pneumoniae strain Klebsiella pneumoniae 459 isolate 459

Toxin (Protein)


Gene name vapC Uniprot ID A0A483GDF6
Locus tag A9493_RS09330 Protein ID WP_004187928.1
Coordinates 1845036..1845419 (+) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag A9493_RS09325 Protein ID WP_004150355.1
Coordinates 1844794..1845036 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A9493_RS09300 1840796..1841395 + 600 WP_004151865.1 glucose-1-phosphatase -
A9493_RS09305 1841389..1842249 + 861 WP_004146232.1 virulence factor BrkB family protein -
A9493_RS09310 1842246..1842683 + 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
A9493_RS09315 1842728..1843669 + 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
A9493_RS09320 1843683..1844600 - 918 WP_004187929.1 alpha/beta hydrolase -
A9493_RS09325 1844794..1845036 + 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
A9493_RS09330 1845036..1845419 + 384 WP_004187928.1 type II toxin-antitoxin system VapC family toxin Toxin
A9493_RS09335 1845593..1846522 - 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
A9493_RS09340 1846519..1847154 - 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
A9493_RS09345 1847151..1848053 - 903 WP_002882822.1 formate dehydrogenase subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-110)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14407.64 Da        Isoelectric Point: 7.3178

>T69671 WP_004187928.1 NZ_CP018306:1845036-1845419 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFTGIPGVVTPYEIHPE

Download         Length: 384 bp

>T69671 NZ_CP018306:1845036-1845419 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTACCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT69671 WP_004150355.1 NZ_CP018306:1844794-1845036 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT69671 NZ_CP018306:1844794-1845036 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A483GDF6

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References