Detailed information of TA system TA069662

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 3166965..3167652
• Replicon: chromosome
• Accession: NZ_CP018305
• Organism: Mycobacterium tuberculosis strain M0018684-2

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P65044
• Locus tag: BOM22_RS15600
• Protein ID: WP_003414624.1
• Coordinates: 3167209..3167652 (+)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: BOM22_RS15595
• Protein ID: WP_009937937.1
• Coordinates: 3166965..3167222 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BOM22_RS15575	3162285..3163139	-	855	WP_003899516.1	GNAT family N-acetyltransferase	-
BOM22_RS15580	3163195..3164358	-	1164	WP_003899517.1	flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase	-
BOM22_RS15585	3164375..3165589	-	1215	WP_003899518.1	zinc metalloprotease Rip	-
BOM22_RS15590	3165597..3166838	-	1242	WP_003414613.1	1-deoxy-D-xylulose-5-phosphate reductoisomerase	-
BOM22_RS15595	3166965..3167222	+	258	WP_009937937.1	ribbon-helix-helix domain-containing protein	Antitoxin
BOM22_RS15600	3167209..3167652	+	444	WP_003414624.1	type II toxin-antitoxin system VapC family toxin	Toxin
BOM22_RS15605	3167732..3168394	+	663	WP_003414630.1	cell surface glycolipoprotein Mpt83	-
BOM22_RS15610	3168490..3168681	+	192	WP_003414632.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
BOM22_RS15615	3169013..3170761	+	1749	WP_003912869.1	cytochrome c biogenesis protein DipZ	-
BOM22_RS15620	3170857..3171438	+	582	WP_003414644.1	fasciclin domain-containing protein	-
BOM22_RS15625	3171538..3171804	+	267	WP_015456449.1	DUF2631 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (1-142)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-38)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16595.72 Da
• Isoelectric Point: 6.4890

>T69662 WP_003414624.1 NZ_CP018305:3167209-3167652 [Mycobacterium tuberculosis]
MLCVDVNVLVYAHRADLREHADYRGLLERLANDDEPLGLPDSVLAGFIRVVTNRRVFTEPTSPQDAWQAVDALLAAPAAM
RLRPGERHWMAFRQLASDVDANGNDIADAHLAAYALENNATWLSADRGFARFRRLRWRHPLDGQTHL

Nucleotide

• Length: 444 bp

>T69662 NZ_CP018305:3167209-3167652 [Mycobacterium tuberculosis]
ATGCTGTGCGTTGATGTCAACGTGCTCGTTTACGCGCATCGGGCAGACCTACGGGAGCACGCGGACTATCGGGGTTTGCT
TGAGCGGCTGGCCAACGATGACGAGCCGCTGGGTCTACCAGATAGCGTGCTCGCCGGCTTCATCCGGGTGGTTACCAACC
GCCGCGTCTTCACCGAGCCGACGAGCCCACAGGACGCATGGCAGGCAGTCGACGCCCTACTCGCGGCACCCGCAGCCATG
CGACTTCGGCCTGGCGAGCGCCACTGGATGGCCTTTCGGCAGTTAGCGTCCGATGTTGATGCGAACGGCAACGACATTGC
GGACGCGCACCTGGCCGCCTACGCGCTAGAGAACAACGCAACCTGGTTGAGCGCCGACCGCGGCTTTGCCCGTTTCCGTC
GACTGCGCTGGCGTCATCCGTTGGACGGTCAGACCCATCTATAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9031.25 Da
• Isoelectric Point: 10.8344

>AT69662 WP_009937937.1 NZ_CP018305:3166965-3167222 [Mycobacterium tuberculosis]
VRTTIRIDDELYREVKAKAARSGRTVAAVLEDAVRRGLNPPKPQAAGRYRVQPSGKGGMRPGVDLSSNAALAEAMNDGVS
VDAVR

Nucleotide

• Length: 258 bp

>AT69662 NZ_CP018305:3166965-3167222 [Mycobacterium tuberculosis]
GTGCGCACGACGATCCGTATCGATGACGAGCTGTACCGCGAGGTGAAAGCAAAGGCCGCTCGTTCCGGGCGTACCGTGGC
CGCGGTTCTTGAAGATGCGGTGCGGCGTGGTCTCAACCCGCCTAAGCCGCAGGCCGCCGGCCGTTATCGAGTCCAGCCGT
CGGGTAAGGGCGGCATGCGGCCCGGTGTCGATCTATCGTCCAACGCCGCACTTGCCGAAGCGATGAACGACGGCGTGTCG
GTCGATGCTGTGCGTTGA

Structures

Toxin

• PDB: 7VWO