69658

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 3094216..3094786 Replicon chromosome
Accession NZ_CP018305
Organism Mycobacterium tuberculosis strain M0018684-2

Toxin (Protein)


Gene name mazF Uniprot ID P71650
Locus tag BOM22_RS15215 Protein ID WP_003414166.1
Coordinates 3094216..3094572 (-) Length 119 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P0CL61
Locus tag BOM22_RS15220 Protein ID WP_003901465.1
Coordinates 3094556..3094786 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BOM22_RS15195 3089668..3091356 - 1689 WP_003414155.1 alpha/beta hydrolase family protein -
BOM22_RS15200 3091360..3091686 - 327 WP_003414157.1 hypothetical protein -
BOM22_RS15205 3091859..3092446 + 588 WP_003914429.1 DUF3558 family protein -
BOM22_RS15210 3092465..3094114 + 1650 WP_003899485.1 CocE/NonD family hydrolase -
BOM22_RS15215 3094216..3094572 - 357 WP_003414166.1 type II toxin-antitoxin system toxin endoribonuclease MazF9 Toxin
BOM22_RS15220 3094556..3094786 - 231 WP_003901465.1 antitoxin MazE Antitoxin
BOM22_RS15225 3094829..3095872 - 1044 WP_003414172.1 DUF2293 domain-containing protein -
BOM22_RS21595 3096063..3096338 + 276 WP_003911993.1 DUF1778 domain-containing protein -
BOM22_RS21600 3096514..3096768 - 255 WP_003917684.1 hypothetical protein -
BOM22_RS15245 3096916..3097320 + 405 WP_009938577.1 hypothetical protein -
BOM22_RS15250 3097317..3097508 + 192 WP_003414184.1 hypothetical protein -
BOM22_RS15255 3097572..3098861 + 1290 Protein_2882 transposase family protein -
BOM22_RS15260 3099095..3099352 + 258 WP_003899489.1 hypothetical protein -
BOM22_RS15265 3099457..3099768 + 312 WP_003414190.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-113)

Antitoxin

Ribbon-helix-helix protein, CopG

(3-29)


Sequences

Toxin        


Download         Length: 119 a.a.        Molecular weight: 12858.73 Da        Isoelectric Point: 9.1962

>T69658 WP_003414166.1 NZ_CP018305:c3094572-3094216 [Mycobacterium tuberculosis]
VMRRGEIWQVDLDPARGSEANNQRPAVVVSNDRANATATRLGRGVITVVPVTSNIAKVYPFQVLLSATTTGLQVDCKAQA
EQIRSIATERLLRPIGRVSAAELAQLDEALKLHLDLWS

Download         Length: 357 bp

>T69658 NZ_CP018305:c3094572-3094216 [Mycobacterium tuberculosis]
GTGATGCGCCGCGGTGAGATTTGGCAGGTCGATCTCGACCCCGCTCGAGGTAGCGAAGCGAACAACCAGCGCCCCGCCGT
CGTCGTCAGCAACGACCGGGCCAACGCGACCGCCACGCGTCTTGGGCGCGGCGTCATCACCGTCGTGCCGGTGACGAGCA
ACATCGCCAAGGTCTATCCGTTTCAGGTGTTGTTGTCGGCCACCACTACTGGTCTCCAGGTCGACTGCAAGGCGCAGGCC
GAGCAAATCAGATCGATTGCTACCGAGCGGTTGCTCCGGCCAATCGGCCGAGTTTCAGCCGCCGAACTTGCCCAGCTCGA
TGAGGCTTTGAAACTGCATCTCGACTTATGGTCGTAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8231.01 Da        Isoelectric Point: 4.1809

>AT69658 WP_003901465.1 NZ_CP018305:c3094786-3094556 [Mycobacterium tuberculosis]
VKLSVSLSDDDVAILDAYVKRAGLPSRSAGLQHAIRVLRYPTLEDDYANAWQEWSAAGDTDAWEQTVGDGVGDAPR

Download         Length: 231 bp

>AT69658 NZ_CP018305:c3094786-3094556 [Mycobacterium tuberculosis]
GTGAAGTTGAGCGTGAGCCTGTCCGATGACGACGTCGCGATCCTGGACGCATATGTGAAACGAGCGGGATTACCATCCCG
CTCCGCCGGCCTGCAGCATGCGATCCGCGTGCTTCGCTACCCCACGCTCGAAGATGACTATGCCAACGCATGGCAAGAAT
GGTCGGCAGCCGGCGACACGGACGCGTGGGAGCAGACCGTCGGCGACGGAGTCGGTGATGCGCCGCGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6L2A
PDB 5HJZ
PDB 6KYT
PDB 6L29
PDB 7DU5
PDB 6KYS

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4FBW3

References