69653

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 2855084..2855721 Replicon chromosome
Accession NZ_CP018305
Organism Mycobacterium tuberculosis strain M0018684-2

Toxin (Protein)


Gene name vapC Uniprot ID P0DMV7
Locus tag BOM22_RS13885 Protein ID WP_003413180.1
Coordinates 2855084..2855479 (-) Length 132 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ44
Locus tag BOM22_RS13890 Protein ID WP_003413183.1
Coordinates 2855476..2855721 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BOM22_RS13840 2850487..2851698 + 1212 WP_009937841.1 alpha/beta hydrolase family protein -
BOM22_RS13850 2851825..2852484 + 660 WP_003900846.1 LppA family lipoprotein -
BOM22_RS13855 2852481..2853143 + 663 WP_003900848.1 hypothetical protein -
BOM22_RS13860 2853140..2853418 + 279 WP_003901422.1 type II toxin-antitoxin system VapB family antitoxin -
BOM22_RS13865 2853511..2853924 + 414 WP_003413164.1 PIN domain nuclease -
BOM22_RS13870 2853963..2854220 + 258 WP_003413167.1 ribbon-helix-helix domain-containing protein -
BOM22_RS13875 2854217..2854594 + 378 WP_003413174.1 type II toxin-antitoxin system VapC family toxin -
BOM22_RS13880 2854610..2854984 - 375 WP_003413177.1 hypothetical protein -
BOM22_RS13885 2855084..2855479 - 396 WP_003413180.1 type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20 Toxin
BOM22_RS13890 2855476..2855721 - 246 WP_003413183.1 type II toxin-antitoxin system antitoxin VapB20 Antitoxin
BOM22_RS13900 2856132..2856551 - 420 WP_003413190.1 A24 family peptidase -
BOM22_RS13905 2856563..2857372 - 810 WP_003413193.1 shikimate dehydrogenase -
BOM22_RS13910 2857369..2858622 - 1254 WP_003413196.1 endolytic transglycosylase MltG -
BOM22_RS13915 2858615..2859127 - 513 WP_003413197.1 Holliday junction resolvase RuvX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Endonuclease VapC20-like

(1-129)

Antitoxin

Ribbon-helix-helix protein, CopG

(11-49)


Sequences

Toxin        


Download         Length: 132 a.a.        Molecular weight: 14619.53 Da        Isoelectric Point: 6.9794

>T69653 WP_003413180.1 NZ_CP018305:c2855479-2855084 [Mycobacterium tuberculosis]
MIFVDTSFWAALGNAGDARHGTAKRLWASKPPVVMTSNHVLGETWTLLNRRCGHRAAVAAAAIRLSTVVRVEHVTADLEE
QAWEWLVRHDEREYSFVDATSFAVMRKKGIQNAYAFDGDFSAAGFVEVRPE

Download         Length: 396 bp

>T69653 NZ_CP018305:c2855479-2855084 [Mycobacterium tuberculosis]
ATGATCTTCGTCGACACGTCCTTCTGGGCTGCACTTGGCAACGCCGGCGACGCTCGGCACGGCACCGCGAAGAGGTTGTG
GGCCAGCAAACCGCCCGTGGTGATGACCTCCAACCATGTCCTGGGCGAGACCTGGACGCTGCTCAACCGGCGCTGCGGTC
ACCGCGCGGCGGTTGCCGCCGCTGCAATCCGCTTGAGCACCGTCGTTCGCGTCGAGCACGTAACAGCCGACCTGGAAGAG
CAGGCATGGGAATGGCTTGTACGCCACGACGAGCGAGAGTACTCGTTCGTCGATGCCACGAGCTTCGCGGTGATGCGAAA
GAAAGGCATCCAAAATGCGTATGCCTTCGATGGTGACTTCAGCGCGGCCGGATTTGTCGAGGTAAGACCCGAGTAG

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9098.33 Da        Isoelectric Point: 5.6007

>AT69653 WP_003413183.1 NZ_CP018305:c2855721-2855476 [Mycobacterium tuberculosis]
MLVAYICHVKRLQIYIDEDVDRALAVEARRRRTSKAALIREYVAEHLRQPGPDPVDAFVGSFVGEADLSASVDDVVYGKH
E

Download         Length: 246 bp

>AT69653 NZ_CP018305:c2855721-2855476 [Mycobacterium tuberculosis]
ATGCTAGTGGCGTATATTTGCCATGTGAAAAGGCTGCAGATCTACATCGACGAAGACGTTGACCGAGCGCTTGCTGTTGA
AGCGCGACGGCGGCGGACGTCGAAGGCGGCGCTGATCCGGGAGTACGTCGCTGAGCACCTCCGGCAGCCGGGCCCAGATC
CGGTCGATGCCTTCGTCGGATCGTTCGTGGGGGAAGCCGACTTGTCCGCGTCCGTCGACGACGTGGTTTACGGAAAGCAC
GAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5WZ4
PDB 5WZF

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4BW31

References