Detailed information of TA system TA069638

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2180070..2180773
• Replicon: chromosome
• Accession: NZ_CP018305
• Organism: Mycobacterium tuberculosis strain M0018684-2

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G0TLK7
• Locus tag: BOM22_RS10480
• Protein ID: WP_003409778.1
• Coordinates: 2180070..2180399 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G0TLK8
• Locus tag: BOM22_RS10485
• Protein ID: WP_003409780.1
• Coordinates: 2180396..2180773 (-)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BOM22_RS10460	2176453..2177523	+	1071	WP_003899091.1	epoxide hydrolase EphB	-
BOM22_RS10465	2177520..2178035	+	516	WP_003409718.1	flavin reductase family protein	-
BOM22_RS10470	2178032..2179093	+	1062	WP_003899092.1	3,4-dihydroxy-2-butanone-4-phosphate synthase	-
BOM22_RS10475	2179090..2179860	+	771	WP_003409775.1	SDR family oxidoreductase	-
BOM22_RS10480	2180070..2180399	-	330	WP_003409778.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
BOM22_RS10485	2180396..2180773	-	378	WP_003409780.1	type II toxin-antitoxin system antitoxin MazE5	Antitoxin
BOM22_RS10490	2180770..2181360	-	591	WP_003409784.1	SEC-C domain-containing protein	-
BOM22_RS10500	2181577..2182838	+	1262	WP_087902221.1	IS3-like element IS987 family transposase	-
BOM22_RS10505	2182881..2184137	+	1257	Protein_1988	HNH endonuclease	-
BOM22_RS10510	2184292..2184744	-	453	WP_003899095.1	lipoprotein	-
BOM22_RS10515	2184808..2185209	+	402	WP_003409869.1	hypothetical protein	-
BOM22_RS10520	2185202..2185384	-	183	WP_003409870.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-103)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 11787.82 Da
• Isoelectric Point: 8.5361

>T69638 WP_003409778.1 NZ_CP018305:c2180399-2180070 [Mycobacterium tuberculosis]
VTALPARGEVWWCEMAEIGRRPVVVLSRDAAIPRLRRALVAPCTTTIRGLASEVVLEPGSDPIPRRSAVNLDSVESVSVA
VLVNRLGRLADIRMRAICTALEVAVDCSR

Nucleotide

• Length: 330 bp

>T69638 NZ_CP018305:c2180399-2180070 [Mycobacterium tuberculosis]
GTGACCGCACTTCCGGCGCGCGGAGAGGTGTGGTGGTGTGAGATGGCTGAGATCGGTCGGCGACCAGTCGTCGTGCTGTC
GCGCGATGCCGCGATCCCTCGGCTGCGACGCGCACTTGTCGCGCCCTGCACCACGACCATCCGAGGGCTAGCCAGTGAGG
TTGTTCTTGAACCCGGTTCCGACCCGATCCCGCGCCGTTCCGCGGTGAATTTGGACTCAGTCGAAAGTGTCTCGGTCGCG
GTATTGGTGAATCGGCTTGGCCGCCTCGCCGACATCCGGATGCGCGCCATCTGCACGGCCCTCGAGGTCGCCGTCGATTG
CTCTCGATGA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13560.07 Da
• Isoelectric Point: 5.1519

>AT69638 WP_003409780.1 NZ_CP018305:c2180773-2180396 [Mycobacterium tuberculosis]
VKTARLQVTLRCAVDLINSSSDQCFARIEHVASDQADPRPGVWHSSGMNRIRLSTTVDAALLTSARDMRAGITDAALIDE
ALAALLARHRSAEVDASYAAYDKHPVDEPDEWGDLASWRRAAGDS

Nucleotide

• Length: 378 bp

>AT69638 NZ_CP018305:c2180773-2180396 [Mycobacterium tuberculosis]
GTGAAGACGGCCCGGTTGCAGGTGACGTTGCGCTGCGCGGTAGACCTCATCAATTCGTCCAGCGACCAATGTTTCGCCCG
TATCGAGCACGTTGCGTCCGATCAGGCGGATCCACGCCCCGGAGTGTGGCATAGTAGTGGCATGAATCGCATACGGCTGA
GCACTACTGTCGACGCCGCACTCTTGACAAGTGCGCGCGACATGCGGGCAGGAATCACTGACGCAGCCCTCATCGATGAG
GCCCTTGCGGCATTACTGGCTCGTCATCGATCGGCCGAGGTGGATGCCAGCTATGCGGCCTACGACAAGCACCCGGTCGA
TGAGCCCGACGAGTGGGGCGATCTAGCGTCGTGGCGGCGGGCGGCTGGAGACTCGTGA

Structures

Toxin

• AlphaFold DB: G0TLK7

Antitoxin

• AlphaFold DB: G0TLK8