Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN-PHD
Location 3783471..3784138 Replicon chromosome
Accession NZ_CP018304
Organism Mycobacterium tuberculosis strain M0002959-6

Toxin (Protein)


Gene name vapC Uniprot ID O50411
Locus tag BOK72_RS18460 Protein ID WP_003417916.1
Coordinates 3783471..3783863 (-) Length 131 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A0E8U8S7
Locus tag BOK72_RS18465 Protein ID WP_003912214.1
Coordinates 3783863..3784138 (-) Length 92 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BOK72_RS18440 3778843..3780679 - 1837 Protein_3482 1-deoxy-D-xylulose-5-phosphate synthase -
BOK72_RS18450 3780676..3781665 - 990 WP_003417912.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
BOK72_RS18455 3781665..3782717 - 1053 WP_003900678.1 polyprenyl synthetase family protein -
BOK72_RS18460 3783471..3783863 - 393 WP_003417916.1 type II toxin-antitoxin system VapC family toxin Toxin
BOK72_RS18465 3783863..3784138 - 276 WP_003912214.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
BOK72_RS21535 3784350..3785691 + 1342 Protein_3487 ISNCY family transposase -
BOK72_RS18480 3785881..3788094 + 2214 WP_070171114.1 PE family protein -
BOK72_RS18485 3788165..3789037 - 873 WP_003417929.1 3-hydroxyacyl-thioester dehydratase HtdY -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 3785014..3785691 677


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(4-120)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-53)


Sequences

Toxin        


Download         Length: 131 a.a.        Molecular weight: 14316.74 Da        Isoelectric Point: 10.7249

>T69605 WP_003417916.1 NZ_CP018304:c3783863-3783471 [Mycobacterium tuberculosis]
MAAIYLDSSAIVKLAVREPESDALRRYLRTRHPRVSSALARAEVMRALLDKGESARKAGRRALAHLDLLRVDKRVLDLAG
GLLPFELRTLDAIHLATAQRLGVDLGRLCTYDDRMRDAAKTLGMAVIAPS

Download         Length: 393 bp

>T69605 NZ_CP018304:c3783863-3783471 [Mycobacterium tuberculosis]
ATGGCGGCCATCTATCTCGACTCGTCCGCGATCGTCAAGCTCGCGGTTCGCGAGCCAGAGTCGGACGCGCTGCGCCGCTA
CCTTCGCACCCGCCACCCGCGAGTGTCGAGTGCCCTTGCGCGCGCGGAGGTCATGCGGGCCCTGCTTGACAAGGGTGAAT
CAGCGCGGAAAGCCGGCCGCCGGGCGCTGGCTCATCTCGATCTGCTGCGCGTCGACAAGCGCGTCCTCGATCTCGCTGGT
GGGCTGCTGCCATTCGAGCTGCGCACGCTCGATGCCATCCACTTGGCGACGGCGCAGCGGCTCGGCGTCGATCTGGGCCG
GTTGTGCACCTACGACGACCGGATGCGCGACGCCGCCAAGACGCTCGGCATGGCCGTGATCGCCCCGTCGTGA

Antitoxin


Download         Length: 92 a.a.        Molecular weight: 9966.35 Da        Isoelectric Point: 4.5332

>AT69605 WP_003912214.1 NZ_CP018304:c3784138-3783863 [Mycobacterium tuberculosis]
MTSVGVRALRQRASELLRRVEAGETIEITDRGRPVALLSPLPQGGPYEQLLASGEIERATLDVVDLPEPLDLDAGVELPS
VTLARLREHER

Download         Length: 276 bp

>AT69605 NZ_CP018304:c3784138-3783863 [Mycobacterium tuberculosis]
ATGACCTCGGTGGGGGTGCGTGCGCTGCGGCAGCGGGCCAGCGAGCTGCTGCGCCGAGTCGAGGCCGGCGAGACGATCGA
GATCACCGACCGCGGCCGGCCCGTCGCCCTGCTCTCGCCGCTGCCGCAGGGCGGTCCATACGAGCAGCTGCTGGCAAGCG
GCGAGATCGAGCGTGCCACGCTCGACGTCGTTGATCTGCCCGAACCGCTCGACCTCGACGCGGGCGTCGAGCTGCCGTCG
GTGACGCTTGCGCGCCTGCGTGAGCACGAGCGTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BXS8

Antitoxin

Source ID Structure
AlphaFold DB A0A0E8U8S7

References