Detailed information of TA system TA069603

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 3693268..3693942
• Replicon: chromosome
• Accession: NZ_CP018304
• Organism: Mycobacterium tuberculosis strain M0002959-6

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P9WF52
• Locus tag: BOK72_RS18120
• Protein ID: WP_003417282.1
• Coordinates: 3693268..3693696 (-)
• Length: 143 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TI59
• Locus tag: BOK72_RS18125
• Protein ID: WP_003417286.1
• Coordinates: 3693700..3693942 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BOK72_RS18095	3689090..3689491	-	402	WP_003417264.1	cytidine deaminase	-
BOK72_RS18100	3689632..3690066	+	435	WP_003900017.1	succinate dehydrogenase, cytochrome b556 subunit	-
BOK72_RS18105	3690063..3690497	+	435	WP_003417269.1	succinate dehydrogenase hydrophobic membrane anchor subunit	-
BOK72_RS18110	3690626..3692398	+	1773	WP_003417273.1	succinate dehydrogenase flavoprotein subunit	-
BOK72_RS18115	3692398..3693189	+	792	WP_003417279.1	succinate dehydrogenase iron-sulfur subunit	-
BOK72_RS18120	3693268..3693696	-	429	WP_003417282.1	type II toxin-antitoxin system VapC family toxin	Toxin
BOK72_RS18125	3693700..3693942	-	243	WP_003417286.1	ribbon-helix-helix domain-containing protein	Antitoxin
BOK72_RS18130	3694064..3694678	-	615	WP_003417288.1	class I SAM-dependent methyltransferase	-
BOK72_RS18135	3694675..3695340	-	666	WP_003417290.1	molybdopterin converting factor subunit 1	-
BOK72_RS18140	3695341..3695874	-	534	WP_003417293.1	cyclic pyranopterin monophosphate synthase MoaC	-
BOK72_RS21765	3695871..3695960	-	90	WP_009934953.1	pterin-4-alpha-carbinolamine dehydratase moaB3	-
BOK72_RS18155	3696059..3697320	+	1262	WP_087902221.1	IS3-like element IS987 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (1-141)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 143 a.a.
• Molecular weight: 15834.16 Da
• Isoelectric Point: 8.5471

>T69603 WP_003417282.1 NZ_CP018304:c3693696-3693268 [Mycobacterium tuberculosis]
MRALLDVNVLLALLDRDHVDHERARAWITGQIERGWASCAITQNGFVRVISQPRYPSPISVAHAIDLLARATHTRYHEFW
SCTVSILDSKVIDRSRLHSPKQVTDAYLLALAVAHDGRFVTFDQSIALTAVPGATKQHLATL

Nucleotide

• Length: 429 bp

>T69603 NZ_CP018304:c3693696-3693268 [Mycobacterium tuberculosis]
ATGCGAGCGCTGTTGGACGTCAACGTGCTACTGGCGCTGTTGGACCGCGATCACGTCGACCACGAGCGCGCCCGAGCGTG
GATCACCGGGCAGATCGAGCGGGGATGGGCTTCCTGTGCGATCACCCAAAACGGGTTCGTCCGCGTCATCAGCCAACCGC
GCTATCCCAGCCCAATCTCGGTAGCCCATGCCATCGACTTGTTGGCTCGTGCGACGCACACGCGCTATCACGAGTTCTGG
TCCTGCACCGTCAGCATTCTCGACTCAAAGGTCATCGATCGTTCCCGCCTGCACAGCCCCAAACAGGTCACCGACGCGTA
CTTGCTGGCCCTCGCGGTCGCTCATGATGGCCGATTTGTCACCTTCGATCAGTCGATTGCGTTGACTGCGGTTCCTGGCG
CAACCAAACAGCACCTGGCAACGCTGTAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8849.86 Da
• Isoelectric Point: 5.1007

>AT69603 WP_003417286.1 NZ_CP018304:c3693942-3693700 [Mycobacterium tuberculosis]
MRTTLSIDDDVLLAVKERARREKRTAGEILSDLARQALTNQNPQPAASQEDAFHGFEPLPHRGGAVSNALIDRLRDEEAV

Nucleotide

• Length: 243 bp

>AT69603 NZ_CP018304:c3693942-3693700 [Mycobacterium tuberculosis]
ATGCGCACCACGTTGTCCATCGACGACGATGTATTGCTGGCCGTGAAGGAACGGGCACGCAGGGAGAAGCGCACCGCGGG
TGAAATCCTGTCGGATCTGGCCCGGCAAGCGTTAACCAACCAGAATCCCCAGCCTGCGGCGAGCCAGGAGGACGCCTTTC
ATGGCTTCGAACCCCTGCCTCATCGCGGTGGAGCGGTATCGAACGCGCTGATCGATCGGCTTCGTGACGAGGAGGCCGTG
TGA

Structures

Toxin

• AlphaFold DB: A0A7U4FBL0

Antitoxin

• AlphaFold DB: G0TI59