Detailed information of TA system TA069599

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN_Sll0205-like-Phd
• Location: 3123864..3124468
• Replicon: chromosome
• Accession: NZ_CP018304
• Organism: Mycobacterium tuberculosis strain M0002959-6

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P71623
• Locus tag: BOK72_RS15395
• Protein ID: WP_003414492.1
• Coordinates: 3123864..3124256 (-)
• Length: 131 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A829CBY8
• Locus tag: BOK72_RS15400
• Protein ID: WP_003414495.1
• Coordinates: 3124253..3124468 (-)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BOK72_RS15365	3119014..3119802	-	789	WP_003917092.1	CRISPR-associated endoribonuclease Cas6	-
BOK72_RS15370	3120136..3120681	-	546	WP_003913758.1	DUF1802 family protein	-
BOK72_RS15375	3120953..3121837	-	885	WP_003414409.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
BOK72_RS15380	3121840..3122727	-	888	WP_003414414.1	type IV toxin-antitoxin system AbiEi family antitoxin	-
BOK72_RS15385	3123032..3123577	-	546	WP_009939112.1	DUF1802 family protein	-
BOK72_RS15390	3123574..3123843	-	270	WP_003414489.1	DUF2277 family protein	-
BOK72_RS15395	3123864..3124256	-	393	WP_003414492.1	type II toxin-antitoxin system VapC family toxin	Toxin
BOK72_RS15400	3124253..3124468	-	216	WP_003414495.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
BOK72_RS15405	3124515..3125264	+	750	WP_003414497.1	enoyl-CoA hydratase	-
BOK72_RS15410	3125343..3126425	-	1083	WP_003414499.1	ABC transporter ATP-binding protein	-
BOK72_RS15415	3126418..3127728	-	1311	WP_003414501.1	ABC transporter substrate-binding protein	-
BOK72_RS15420	3127731..3128558	-	828	WP_003414504.1	carbohydrate ABC transporter permease	-
BOK72_RS15425	3128555..3129466	-	912	WP_003414505.1	sugar ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• VapC-like Sll0205 protein, PIN domain (5-130)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (2-46)

Sequences

Toxin

Protein

• Length: 131 a.a.
• Molecular weight: 14610.74 Da
• Isoelectric Point: 6.7594

>T69599 WP_003414492.1 NZ_CP018304:c3124256-3123864 [Mycobacterium tuberculosis]
MTTVLLDSHVAYWWSAEPQRLSMAASQAIEHADELAVAAISWFELAWLAEQERIQLAIPVLSWLQQLAEHVRTVGITPSV
AATAVALPSSFPGDPADRLIYATAIEHGWRLVTKDRRLRSHRHPRPVTVW

Nucleotide

• Length: 393 bp

>T69599 NZ_CP018304:c3124256-3123864 [Mycobacterium tuberculosis]
ATGACGACGGTGCTGCTCGACTCGCATGTGGCCTACTGGTGGTCGGCCGAGCCGCAGCGTCTCAGCATGGCGGCGAGCCA
GGCCATCGAACACGCCGACGAGCTCGCCGTCGCCGCGATTTCGTGGTTCGAGCTGGCTTGGCTTGCCGAACAGGAACGCA
TCCAACTGGCGATTCCGGTGCTGTCCTGGCTTCAGCAGCTGGCCGAGCACGTTCGCACCGTCGGTATCACGCCCTCGGTC
GCCGCCACGGCGGTGGCGCTGCCCTCGTCGTTCCCCGGCGATCCGGCCGACCGGTTGATCTACGCCACCGCGATCGAACA
CGGCTGGCGGCTGGTGACCAAGGACCGGCGGCTACGCAGTCATCGGCACCCACGACCGGTCACCGTCTGGTAG

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 7511.55 Da
• Isoelectric Point: 4.9732

>AT69599 WP_003414495.1 NZ_CP018304:c3124468-3124253 [Mycobacterium tuberculosis]
MTATEVKAKILSLLDEVAQGEEIEITKHGRTVARLVAATGPHALKGRFSGVAMAAVDDDELFTTGVSWNVS

Nucleotide

• Length: 216 bp

>AT69599 NZ_CP018304:c3124468-3124253 [Mycobacterium tuberculosis]
ATGACCGCTACGGAGGTGAAGGCGAAGATCCTCTCCTTGCTTGATGAAGTGGCCCAGGGCGAGGAGATCGAGATCACCAA
ACACGGCCGCACCGTGGCCCGGCTGGTGGCAGCGACGGGGCCGCACGCGCTGAAGGGTCGATTCTCGGGTGTGGCGATGG
CGGCCGTGGATGACGACGAACTCTTCACCACCGGGGTTTCGTGGAACGTTTCATGA

Structures

Toxin

• AlphaFold DB: A0A7U4BWM8

Antitoxin

• AlphaFold DB: A0A829CBY8