Detailed information of TA system TA069595

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 3057694..3058373
• Replicon: chromosome
• Accession: NZ_CP018304
• Organism: Mycobacterium tuberculosis strain M0002959-6

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P9WF90
• Locus tag: BOK72_RS15015
• Protein ID: WP_003414059.1
• Coordinates: 3057694..3058110 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TF64
• Locus tag: BOK72_RS15020
• Protein ID: WP_003414061.1
• Coordinates: 3058107..3058373 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BOK72_RS14995	3053746..3054648	-	903	WP_003900564.1	4-hydroxy-tetrahydrodipicolinate synthase	-
BOK72_RS15000	3054717..3055469	-	753	WP_003899465.1	FAD-dependent thymidylate synthase	-
BOK72_RS15005	3055713..3055988	-	276	WP_003414055.1	type I restriction endonuclease subunit S	-
BOK72_RS15010	3055985..3057607	-	1623	WP_003906897.1	type I restriction-modification system subunit M	-
BOK72_RS15015	3057694..3058110	-	417	WP_003414059.1	type II toxin-antitoxin system toxin ribonuclease C21	Toxin
BOK72_RS15020	3058107..3058373	-	267	WP_003414061.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
BOK72_RS15025	3058399..3058794	-	396	WP_003414064.1	type II toxin-antitoxin system VapC family toxin	-
BOK72_RS15030	3058791..3059060	-	270	WP_003414066.1	type II toxin-antitoxin system VapB family antitoxin	-
BOK72_RS15035	3059070..3060164	-	1095	WP_003414068.1	restriction endonuclease subunit S	-
BOK72_RS15040	3060161..3060580	-	420	WP_003414070.1	winged helix-turn-helix domain-containing protein	-
BOK72_RS15045	3060654..3061133	-	480	WP_003414073.1	dihydrofolate reductase	-
BOK72_RS15050	3061204..3062004	-	801	WP_009939104.1	thymidylate synthase	-
BOK72_RS15055	3062160..3062897	+	738	WP_003414079.1	dienelactone hydrolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-123)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (16-56)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15773.00 Da
• Isoelectric Point: 7.1294

>T69595 WP_003414059.1 NZ_CP018304:c3058110-3057694 [Mycobacterium tuberculosis]
MTTRYLLDKSAAYRAHLPAVRHRLEPLMERGLLARCGITDLEFGVSARSREDHRTLGTYRRDALEYVNTPDTVWVRAWEI
QEALTDKGFHRSVKIPDLIIAAVAEHHGIPVMHYDQDFERIAAITRQPVEWVVAPGTA

Nucleotide

• Length: 417 bp

>T69595 NZ_CP018304:c3058110-3057694 [Mycobacterium tuberculosis]
ATGACCACGCGCTATTTGCTCGACAAATCAGCGGCTTACCGCGCGCACTTGCCCGCGGTCCGACATCGCTTGGAACCGTT
GATGGAACGCGGTCTACTGGCCCGGTGCGGCATTACCGATCTCGAGTTCGGAGTCTCGGCGCGTTCCCGCGAGGACCATC
GAACACTGGGCACCTACCGGCGTGACGCGCTCGAATACGTCAACACCCCCGACACCGTGTGGGTTCGTGCATGGGAGATC
CAAGAAGCATTGACCGACAAGGGATTTCACCGCTCGGTCAAGATCCCGGACTTGATCATTGCGGCGGTCGCCGAGCATCA
CGGCATACCCGTCATGCACTACGACCAAGACTTCGAACGCATCGCCGCCATCACACGGCAACCCGTGGAATGGGTGGTGG
CCCCAGGGACAGCGTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9493.77 Da
• Isoelectric Point: 7.2783

>AT69595 WP_003414061.1 NZ_CP018304:c3058373-3058107 [Mycobacterium tuberculosis]
MHRGYALVVCSPGVTRTMIDIDDDLLARAAKELGTTTKKDTVHAALRAALRASAARSLMNRMAENATGTQDEALVNAMWR
DGHPENTA

Nucleotide

• Length: 267 bp

>AT69595 NZ_CP018304:c3058373-3058107 [Mycobacterium tuberculosis]
ATGCATCGCGGATATGCATTAGTAGTATGCTCTCCGGGTGTGACGCGCACCATGATCGACATCGATGACGACCTGCTCGC
TCGGGCCGCCAAGGAACTGGGTACCACGACAAAGAAAGACACCGTGCATGCCGCGCTGCGGGCCGCGTTGCGAGCCAGTG
CCGCGCGCTCGCTCATGAACCGGATGGCCGAGAACGCCACCGGAACCCAGGACGAAGCTCTGGTCAACGCAATGTGGCGA
GACGGCCACCCAGAGAACACGGCATGA

Structures

Toxin

• PDB: 5SV2

Antitoxin

• AlphaFold DB: G0TF64