Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 2857489..2858120 Replicon chromosome
Accession NZ_CP018304
Organism Mycobacterium tuberculosis strain M0002959-6

Toxin (Protein)


Gene name vapC Uniprot ID G0TQ28
Locus tag BOK72_RS13895 Protein ID WP_003413174.1
Coordinates 2857743..2858120 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P95006
Locus tag BOK72_RS13890 Protein ID WP_003413167.1
Coordinates 2857489..2857746 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BOK72_RS13855 2853403..2853717 + 315 WP_009937839.1 hypothetical protein -
BOK72_RS13860 2854013..2855224 + 1212 WP_003900845.1 alpha/beta hydrolase family protein -
BOK72_RS13870 2855351..2856010 + 660 WP_003900846.1 LppA family lipoprotein -
BOK72_RS13875 2856007..2856669 + 663 WP_003900848.1 hypothetical protein -
BOK72_RS13880 2856666..2856944 + 279 WP_003901422.1 type II toxin-antitoxin system VapB family antitoxin -
BOK72_RS13885 2857037..2857450 + 414 WP_003413164.1 PIN domain nuclease -
BOK72_RS13890 2857489..2857746 + 258 WP_003413167.1 ribbon-helix-helix domain-containing protein Antitoxin
BOK72_RS13895 2857743..2858120 + 378 WP_003413174.1 type II toxin-antitoxin system VapC family toxin Toxin
BOK72_RS13900 2858136..2858510 - 375 WP_003413177.1 hypothetical protein -
BOK72_RS13905 2858610..2859005 - 396 WP_003413180.1 type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20 -
BOK72_RS13910 2859002..2859247 - 246 WP_003413183.1 type II toxin-antitoxin system antitoxin VapB20 -
BOK72_RS13920 2859658..2860077 - 420 WP_003413190.1 A24 family peptidase -
BOK72_RS13925 2860089..2860898 - 810 WP_003413193.1 shikimate dehydrogenase -
BOK72_RS13930 2860895..2862148 - 1254 WP_003413196.1 endolytic transglycosylase MltG -
BOK72_RS13935 2862141..2862653 - 513 WP_003413197.1 Holliday junction resolvase RuvX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(3-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(2-38)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 13728.72 Da        Isoelectric Point: 4.4687

>T69591 WP_003413174.1 NZ_CP018304:2857743-2858120 [Mycobacterium tuberculosis]
VKLIDTTIAVDHLRGEPAAAVLLAELINNGEEIAASELVRFELLAGVRESELAALEAFFSAVVWTLVTEDIARIGGRLAR
RYRSSHRGIDDVDYLIAATAIVVDADLLTTNVRHFPMFPDLQPPY

Download         Length: 378 bp

>T69591 NZ_CP018304:2857743-2858120 [Mycobacterium tuberculosis]
GTGAAGCTGATCGACACCACCATCGCGGTCGACCACCTTCGCGGCGAACCCGCGGCAGCCGTGCTGCTCGCCGAACTGAT
AAACAACGGTGAGGAGATCGCGGCCAGCGAGCTGGTCCGATTCGAACTCCTCGCCGGTGTGCGGGAAAGCGAACTCGCGG
CGCTCGAGGCCTTCTTCTCGGCAGTGGTGTGGACCCTGGTGACCGAGGACATTGCCCGGATCGGCGGACGACTCGCCCGT
CGATACCGGTCCAGCCACCGCGGTATCGACGACGTGGACTACCTGATCGCTGCGACCGCCATTGTGGTCGACGCCGACCT
GCTCACCACCAATGTGCGCCACTTCCCGATGTTCCCGGATCTGCAGCCGCCGTACTGA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9453.63 Da        Isoelectric Point: 10.6555

>AT69591 WP_003413167.1 NZ_CP018304:2857489-2857746 [Mycobacterium tuberculosis]
MRTQVTLGKEELELLDRAAKASGASRSELIRRAIHRAYGTGSKQERLAALDHSRGSWRGRDFTGTEYVDAIRGDLNERLA
RLGLA

Download         Length: 258 bp

>AT69591 NZ_CP018304:2857489-2857746 [Mycobacterium tuberculosis]
ATGCGGACTCAGGTGACCCTGGGCAAAGAGGAGCTTGAGCTGCTCGATCGTGCCGCCAAGGCGAGTGGCGCATCGCGGTC
CGAACTCATCCGACGCGCAATTCACCGTGCCTACGGGACTGGATCCAAGCAGGAACGGCTCGCCGCGCTCGACCACAGCC
GTGGCTCGTGGCGAGGACGGGACTTCACCGGCACCGAGTATGTCGACGCCATTCGGGGCGACCTCAACGAACGACTTGCT
CGGCTCGGTCTGGCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G0TQ28

Antitoxin

Source ID Structure
AlphaFold DB A0A829C9W5

References