Detailed information of TA system TA069591

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 2857489..2858120
• Replicon: chromosome
• Accession: NZ_CP018304
• Organism: Mycobacterium tuberculosis strain M0002959-6

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ28
• Locus tag: BOK72_RS13895
• Protein ID: WP_003413174.1
• Coordinates: 2857743..2858120 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P95006
• Locus tag: BOK72_RS13890
• Protein ID: WP_003413167.1
• Coordinates: 2857489..2857746 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BOK72_RS13855	2853403..2853717	+	315	WP_009937839.1	hypothetical protein	-
BOK72_RS13860	2854013..2855224	+	1212	WP_003900845.1	alpha/beta hydrolase family protein	-
BOK72_RS13870	2855351..2856010	+	660	WP_003900846.1	LppA family lipoprotein	-
BOK72_RS13875	2856007..2856669	+	663	WP_003900848.1	hypothetical protein	-
BOK72_RS13880	2856666..2856944	+	279	WP_003901422.1	type II toxin-antitoxin system VapB family antitoxin	-
BOK72_RS13885	2857037..2857450	+	414	WP_003413164.1	PIN domain nuclease	-
BOK72_RS13890	2857489..2857746	+	258	WP_003413167.1	ribbon-helix-helix domain-containing protein	Antitoxin
BOK72_RS13895	2857743..2858120	+	378	WP_003413174.1	type II toxin-antitoxin system VapC family toxin	Toxin
BOK72_RS13900	2858136..2858510	-	375	WP_003413177.1	hypothetical protein	-
BOK72_RS13905	2858610..2859005	-	396	WP_003413180.1	type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20	-
BOK72_RS13910	2859002..2859247	-	246	WP_003413183.1	type II toxin-antitoxin system antitoxin VapB20	-
BOK72_RS13920	2859658..2860077	-	420	WP_003413190.1	A24 family peptidase	-
BOK72_RS13925	2860089..2860898	-	810	WP_003413193.1	shikimate dehydrogenase	-
BOK72_RS13930	2860895..2862148	-	1254	WP_003413196.1	endolytic transglycosylase MltG	-
BOK72_RS13935	2862141..2862653	-	513	WP_003413197.1	Holliday junction resolvase RuvX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-38)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13728.72 Da
• Isoelectric Point: 4.4687

>T69591 WP_003413174.1 NZ_CP018304:2857743-2858120 [Mycobacterium tuberculosis]
VKLIDTTIAVDHLRGEPAAAVLLAELINNGEEIAASELVRFELLAGVRESELAALEAFFSAVVWTLVTEDIARIGGRLAR
RYRSSHRGIDDVDYLIAATAIVVDADLLTTNVRHFPMFPDLQPPY

Nucleotide

• Length: 378 bp

>T69591 NZ_CP018304:2857743-2858120 [Mycobacterium tuberculosis]
GTGAAGCTGATCGACACCACCATCGCGGTCGACCACCTTCGCGGCGAACCCGCGGCAGCCGTGCTGCTCGCCGAACTGAT
AAACAACGGTGAGGAGATCGCGGCCAGCGAGCTGGTCCGATTCGAACTCCTCGCCGGTGTGCGGGAAAGCGAACTCGCGG
CGCTCGAGGCCTTCTTCTCGGCAGTGGTGTGGACCCTGGTGACCGAGGACATTGCCCGGATCGGCGGACGACTCGCCCGT
CGATACCGGTCCAGCCACCGCGGTATCGACGACGTGGACTACCTGATCGCTGCGACCGCCATTGTGGTCGACGCCGACCT
GCTCACCACCAATGTGCGCCACTTCCCGATGTTCCCGGATCTGCAGCCGCCGTACTGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9453.63 Da
• Isoelectric Point: 10.6555

>AT69591 WP_003413167.1 NZ_CP018304:2857489-2857746 [Mycobacterium tuberculosis]
MRTQVTLGKEELELLDRAAKASGASRSELIRRAIHRAYGTGSKQERLAALDHSRGSWRGRDFTGTEYVDAIRGDLNERLA
RLGLA

Nucleotide

• Length: 258 bp

>AT69591 NZ_CP018304:2857489-2857746 [Mycobacterium tuberculosis]
ATGCGGACTCAGGTGACCCTGGGCAAAGAGGAGCTTGAGCTGCTCGATCGTGCCGCCAAGGCGAGTGGCGCATCGCGGTC
CGAACTCATCCGACGCGCAATTCACCGTGCCTACGGGACTGGATCCAAGCAGGAACGGCTCGCCGCGCTCGACCACAGCC
GTGGCTCGTGGCGAGGACGGGACTTCACCGGCACCGAGTATGTCGACGCCATTCGGGGCGACCTCAACGAACGACTTGCT
CGGCTCGGTCTGGCGTGA

Structures

Toxin

• AlphaFold DB: G0TQ28

Antitoxin

• AlphaFold DB: A0A829C9W5