Detailed information of TA system TA069577

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2181885..2182588
• Replicon: chromosome
• Accession: NZ_CP018304
• Organism: Mycobacterium tuberculosis strain M0002959-6

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G0TLK7
• Locus tag: BOK72_RS10495
• Protein ID: WP_003409778.1
• Coordinates: 2181885..2182214 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G0TLK8
• Locus tag: BOK72_RS10500
• Protein ID: WP_003409780.1
• Coordinates: 2182211..2182588 (-)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BOK72_RS10475	2178268..2179338	+	1071	WP_003899091.1	epoxide hydrolase EphB	-
BOK72_RS10480	2179335..2179850	+	516	WP_003409718.1	flavin reductase family protein	-
BOK72_RS10485	2179847..2180908	+	1062	WP_003899092.1	3,4-dihydroxy-2-butanone-4-phosphate synthase	-
BOK72_RS10490	2180905..2181675	+	771	WP_003409775.1	SDR family oxidoreductase	-
BOK72_RS10495	2181885..2182214	-	330	WP_003409778.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
BOK72_RS10500	2182211..2182588	-	378	WP_003409780.1	type II toxin-antitoxin system antitoxin MazE5	Antitoxin
BOK72_RS10505	2182585..2183175	-	591	WP_003409784.1	SEC-C domain-containing protein	-
BOK72_RS10510	2183230..2184594	+	1365	WP_003903691.1	HNH endonuclease	-
BOK72_RS10515	2184749..2185201	-	453	WP_003899095.1	lipoprotein	-
BOK72_RS10520	2185265..2185666	+	402	WP_003409869.1	hypothetical protein	-
BOK72_RS10525	2185659..2185841	-	183	WP_003409870.1	hypothetical protein	-
BOK72_RS10530	2185955..2186305	-	351	WP_003899096.1	hypothetical protein	-
BOK72_RS10535	2186316..2187218	-	903	WP_003899097.1	hypothetical protein	-
BOK72_RS10540	2187239..2187430	-	192	WP_003409876.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-103)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 11787.82 Da
• Isoelectric Point: 8.5361

>T69577 WP_003409778.1 NZ_CP018304:c2182214-2181885 [Mycobacterium tuberculosis]
VTALPARGEVWWCEMAEIGRRPVVVLSRDAAIPRLRRALVAPCTTTIRGLASEVVLEPGSDPIPRRSAVNLDSVESVSVA
VLVNRLGRLADIRMRAICTALEVAVDCSR

Nucleotide

• Length: 330 bp

>T69577 NZ_CP018304:c2182214-2181885 [Mycobacterium tuberculosis]
GTGACCGCACTTCCGGCGCGCGGAGAGGTGTGGTGGTGTGAGATGGCTGAGATCGGTCGGCGACCAGTCGTCGTGCTGTC
GCGCGATGCCGCGATCCCTCGGCTGCGACGCGCACTTGTCGCGCCCTGCACCACGACCATCCGAGGGCTAGCCAGTGAGG
TTGTTCTTGAACCCGGTTCCGACCCGATCCCGCGCCGTTCCGCGGTGAATTTGGACTCAGTCGAAAGTGTCTCGGTCGCG
GTATTGGTGAATCGGCTTGGCCGCCTCGCCGACATCCGGATGCGCGCCATCTGCACGGCCCTCGAGGTCGCCGTCGATTG
CTCTCGATGA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13560.07 Da
• Isoelectric Point: 5.1519

>AT69577 WP_003409780.1 NZ_CP018304:c2182588-2182211 [Mycobacterium tuberculosis]
VKTARLQVTLRCAVDLINSSSDQCFARIEHVASDQADPRPGVWHSSGMNRIRLSTTVDAALLTSARDMRAGITDAALIDE
ALAALLARHRSAEVDASYAAYDKHPVDEPDEWGDLASWRRAAGDS

Nucleotide

• Length: 378 bp

>AT69577 NZ_CP018304:c2182588-2182211 [Mycobacterium tuberculosis]
GTGAAGACGGCCCGGTTGCAGGTGACGTTGCGCTGCGCGGTAGACCTCATCAATTCGTCCAGCGACCAATGTTTCGCCCG
TATCGAGCACGTTGCGTCCGATCAGGCGGATCCACGCCCCGGAGTGTGGCATAGTAGTGGCATGAATCGCATACGGCTGA
GCACTACTGTCGACGCCGCACTCTTGACAAGTGCGCGCGACATGCGGGCAGGAATCACTGACGCAGCCCTCATCGATGAG
GCCCTTGCGGCATTACTGGCTCGTCATCGATCGGCCGAGGTGGATGCCAGCTATGCGGCCTACGACAAGCACCCGGTCGA
TGAGCCCGACGAGTGGGGCGATCTAGCGTCGTGGCGGCGGGCGGCTGGAGACTCGTGA

Structures

Toxin

• AlphaFold DB: G0TLK7

Antitoxin

• AlphaFold DB: G0TLK8