Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 1936417..1937030 Replicon chromosome
Accession NZ_CP018304
Organism Mycobacterium tuberculosis strain M0002959-6

Toxin (Protein)


Gene name vapC Uniprot ID P9WFA2
Locus tag BOK72_RS09320 Protein ID WP_003408465.1
Coordinates 1936417..1936806 (-) Length 130 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ52
Locus tag BOK72_RS09325 Protein ID WP_003408469.1
Coordinates 1936803..1937030 (-) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BOK72_RS09285 1932047..1932961 + 915 WP_003898985.1 3-hydroxyacyl-CoA dehydrogenase family protein -
BOK72_RS09290 1932964..1933794 + 831 WP_003906661.1 cyclase family protein -
BOK72_RS09295 1933794..1934143 + 350 Protein_1761 cupin domain-containing protein -
BOK72_RS09300 1934196..1935014 + 819 WP_003408456.1 3-keto-5-aminohexanoate cleavage protein -
BOK72_RS09305 1935028..1935807 + 780 WP_003408460.1 IclR family transcriptional regulator -
BOK72_RS09320 1936417..1936806 - 390 WP_003408465.1 PIN domain-containing protein Toxin
BOK72_RS09325 1936803..1937030 - 228 WP_003408469.1 antitoxin Antitoxin
BOK72_RS09330 1937248..1938732 + 1485 WP_009937637.1 biotin carboxylase -
BOK72_RS09335 1938729..1939976 + 1248 WP_003408476.1 serine hydrolase -
BOK72_RS09340 1940019..1940438 - 420 WP_003408483.1 hypothetical protein -
BOK72_RS09345 1940428..1941138 - 711 WP_003408486.1 winged helix-turn-helix transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-116)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 130 a.a.        Molecular weight: 13817.98 Da        Isoelectric Point: 7.0612

>T69575 WP_003408465.1 NZ_CP018304:c1936806-1936417 [Mycobacterium tuberculosis]
VIVLDASAAVELMLTTPAGAAVARRLRGETVHAPAHFDVEVIGAIRQAVVRQLISDHEGLVVVVNFLSLPVRRWPLKPFT
QRAYQLRSTHTVADGAYVALAEGLGVPLITCDGRLAQSHGHNAEIELVA

Download         Length: 390 bp

>T69575 NZ_CP018304:c1936806-1936417 [Mycobacterium tuberculosis]
GTGATCGTGTTGGACGCCTCGGCGGCCGTCGAGCTCATGCTCACGACGCCGGCGGGTGCGGCGGTCGCCCGGCGCTTGCG
GGGCGAAACCGTGCACGCCCCTGCACATTTCGATGTCGAGGTGATCGGGGCGATACGTCAAGCGGTCGTGCGTCAGCTGA
TCAGCGATCATGAAGGACTCGTCGTGGTTGTCAACTTCCTTAGCCTGCCCGTTCGGCGTTGGCCGTTAAAGCCATTCACC
CAGCGTGCCTACCAACTTCGGTCCACTCATACCGTGGCCGATGGAGCCTACGTCGCCCTCGCCGAGGGCCTCGGCGTACC
GCTTATCACTTGCGACGGGCGACTTGCCCAATCCCATGGCCATAATGCGGAGATCGAGCTTGTCGCCTGA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8226.39 Da        Isoelectric Point: 4.3313

>AT69575 WP_003408469.1 NZ_CP018304:c1937030-1936803 [Mycobacterium tuberculosis]
MSAMVQIRNVPDELLHELKARAAAQRMSLSDFLLARLAEIAEEPALDDVLDRLAALPRRDLGASAAELVDEARSE

Download         Length: 228 bp

>AT69575 NZ_CP018304:c1937030-1936803 [Mycobacterium tuberculosis]
ATGTCCGCCATGGTTCAGATCCGCAACGTTCCCGACGAGCTTCTCCACGAGCTGAAGGCCCGCGCAGCGGCGCAACGGAT
GAGCCTGAGCGATTTCCTCCTAGCACGGCTTGCCGAGATCGCGGAAGAACCGGCCCTCGACGACGTTCTCGACCGTCTAG
CGGCACTGCCGCGCCGGGATCTCGGCGCGAGCGCAGCCGAGCTTGTTGACGAGGCTCGATCTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BUI9

Antitoxin

Source ID Structure
PDB 7E4J
AlphaFold DB A0A7U4FAN9

References