Detailed information of TA system TA069568

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1237965..1238533
• Replicon: chromosome
• Accession: NZ_CP018304
• Organism: Mycobacterium tuberculosis strain M0002959-6

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TH08
• Locus tag: BOK72_RS06120
• Protein ID: WP_003405865.1
• Coordinates: 1238159..1238533 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TH07
• Locus tag: BOK72_RS06115
• Protein ID: WP_003405863.1
• Coordinates: 1237965..1238162 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BOK72_RS06095	1234006..1234644	-	639	WP_003898730.1	lipid droplet-associated protein	-
BOK72_RS06100	1234734..1235741	+	1008	WP_003405853.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
BOK72_RS06105	1235758..1236741	-	984	WP_003898731.1	hypothetical protein	-
BOK72_RS06110	1236804..1237877	+	1074	WP_003898732.1	redox-regulated ATPase YchF	-
BOK72_RS06115	1237965..1238162	+	198	WP_003405863.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
BOK72_RS06120	1238159..1238533	+	375	WP_003405865.1	type II toxin-antitoxin system VapC family toxin	Toxin
BOK72_RS06125	1238736..1239434	+	699	WP_003898733.1	hypothetical protein	-
BOK72_RS06130	1239552..1239737	+	186	WP_003901093.1	hypothetical protein	-
BOK72_RS06135	1239664..1239939	-	276	WP_003405867.1	hypothetical protein	-
BOK72_RS06140	1240182..1240505	+	324	WP_003405871.1	antibiotic biosynthesis monooxygenase	-
BOK72_RS06145	1240520..1241230	-	711	WP_003911399.1	hypothetical protein	-
BOK72_RS21910	1241252..1241461	+	210	WP_003911400.1	hypothetical protein	-
BOK72_RS06155	1241413..1242120	-	708	Protein_1165	adenylate/guanylate cyclase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-111)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13444.56 Da
• Isoelectric Point: 5.1881

>T69568 WP_003405865.1 NZ_CP018304:1238159-1238533 [Mycobacterium tuberculosis]
VILVDTSVWIEHLRAADARLVELLGDDEAGCHPLVIEELALGSIKQRDVVLDLLANLYQFPVVTHDEVLRLVGRRRLWGR
GLGAVDANLLGSVALVGGARLWTRDKRLKAACAESGVALAEEVS

Nucleotide

• Length: 375 bp

>T69568 NZ_CP018304:1238159-1238533 [Mycobacterium tuberculosis]
GTGATCCTGGTCGACACTTCGGTATGGATTGAGCACCTGCGCGCCGCCGACGCGCGACTCGTCGAGCTGCTGGGCGATGA
CGAGGCCGGTTGCCATCCGCTCGTCATCGAGGAGCTGGCGCTTGGCTCGATCAAGCAGCGAGACGTTGTTCTCGATCTGT
TGGCCAACCTCTACCAGTTTCCGGTGGTGACCCACGACGAAGTGTTGCGGCTTGTCGGTCGGCGGCGGTTGTGGGGTCGG
GGACTCGGTGCCGTCGATGCCAACCTTCTTGGTTCGGTGGCTCTGGTTGGCGGCGCGCGACTATGGACGCGGGACAAGCG
GTTGAAGGCGGCGTGCGCGGAAAGCGGTGTTGCGCTGGCTGAGGAAGTGTCCTGA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 6973.03 Da
• Isoelectric Point: 11.7327

>AT69568 WP_003405863.1 NZ_CP018304:1237965-1238162 [Mycobacterium tuberculosis]
MRTTVTVDDALLAKAAELTGVKEKSTLLREGLQTLVRVESARRLAALGGTDPQATAAPRRRTSPR

Nucleotide

• Length: 198 bp

>AT69568 NZ_CP018304:1237965-1238162 [Mycobacterium tuberculosis]
ATGAGGACGACGGTGACCGTTGACGACGCCTTGTTAGCCAAAGCGGCCGAATTGACTGGGGTGAAAGAGAAGTCGACGCT
CCTGCGCGAGGGGTTGCAGACACTGGTCCGGGTGGAGAGCGCCCGGCGGTTGGCGGCTCTCGGCGGCACCGACCCGCAAG
CTACCGCGGCGCCGAGACGCCGGACGTCGCCCCGGTGA

Structures

Toxin

• AlphaFold DB: G0TH08

Antitoxin

• AlphaFold DB: G0TH07