Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1-PHD
Location 691857..692503 Replicon chromosome
Accession NZ_CP018304
Organism Mycobacterium tuberculosis strain M0002959-6

Toxin (Protein)


Gene name vapC Uniprot ID O07783
Locus tag BOK72_RS03155 Protein ID WP_003403122.1
Coordinates 691857..692249 (-) Length 131 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TQ86
Locus tag BOK72_RS03160 Protein ID WP_003403125.1
Coordinates 692246..692503 (-) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BOK72_RS03140 687519..689045 + 1527 WP_003403112.1 virulence factor Mce family protein -
BOK72_RS03145 689108..690250 + 1143 WP_003911253.1 virulence factor Mce family protein -
BOK72_RS03150 690255..691805 + 1551 WP_003403119.1 MCE family protein -
BOK72_RS03155 691857..692249 - 393 WP_003403122.1 type II toxin-antitoxin system toxin ribonuclease C4 Toxin
BOK72_RS03160 692246..692503 - 258 WP_003403125.1 type II toxin-antitoxin system antitoxin VapB4 Antitoxin
BOK72_RS03165 692686..693921 - 1236 WP_003403128.1 ATP-binding protein -
BOK72_RS03170 694172..694585 - 414 WP_003403137.1 type II toxin-antitoxin system VapC family toxin -
BOK72_RS03175 694582..694818 - 237 WP_003403139.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
BOK72_RS03180 694922..695428 - 507 WP_003900973.1 two component system sensor kinase HK1 -
BOK72_RS03185 695542..696072 - 531 WP_003917320.1 two component system sensor kinase HK2 -
BOK72_RS03190 696056..696817 - 762 WP_003403148.1 two-component system response regulator TcrA -
BOK72_RS03195 696874..697185 + 312 WP_003403164.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(8-122)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(3-53)


Sequences

Toxin        


Download         Length: 131 a.a.        Molecular weight: 14080.08 Da        Isoelectric Point: 4.6558

>T69553 WP_003403122.1 NZ_CP018304:c692249-691857 [Mycobacterium tuberculosis]
VNVRRALADTSVFIGIEATRFDPDRFAGYEWGVSVVTLGELRLGVLQASGPEAAARRLSTYQLAQRFEPLGIDEAVSEAW
ALLVSKLRAAKLRVPINDSWIAATAVAHGIAILTQDNDYAAMPDVEVITI

Download         Length: 393 bp

>T69553 NZ_CP018304:c692249-691857 [Mycobacterium tuberculosis]
GTGAACGTACGGCGCGCCCTCGCCGACACATCCGTTTTCATTGGCATCGAGGCGACACGATTCGACCCGGACCGGTTTGC
GGGATACGAATGGGGCGTCTCGGTAGTCACACTCGGCGAGCTGCGGCTCGGGGTGTTGCAAGCCTCCGGCCCCGAGGCCG
CCGCACGTCGGCTCTCCACCTACCAGCTTGCGCAACGGTTCGAACCACTAGGCATCGACGAGGCGGTCTCCGAAGCATGG
GCATTACTGGTGTCCAAGCTGCGCGCCGCCAAACTGCGCGTGCCGATCAACGACAGCTGGATTGCGGCCACAGCCGTGGC
GCACGGCATTGCGATCCTGACCCAAGACAACGACTACGCCGCCATGCCCGACGTCGAGGTCATAACGATCTGA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9681.06 Da        Isoelectric Point: 7.0175

>AT69553 WP_003403125.1 NZ_CP018304:c692503-692246 [Mycobacterium tuberculosis]
MSATIPARDLRNHTAEVLRRVAAGEEIEVLKDNRPVARIVPLKRRRQWLPAAEVIGELVRLGPDTTNLGEELRETLTQTT
DDVRW

Download         Length: 258 bp

>AT69553 NZ_CP018304:c692503-692246 [Mycobacterium tuberculosis]
ATGTCTGCTACGATACCCGCTCGCGATCTTCGCAACCACACCGCCGAGGTACTGCGGCGAGTTGCCGCCGGCGAGGAAAT
CGAGGTGCTCAAGGACAATCGCCCCGTAGCGCGCATCGTTCCGCTCAAGCGGCGCCGCCAATGGTTGCCAGCTGCCGAGG
TGATCGGCGAACTGGTGCGCTTGGGCCCCGATACCACCAATCTGGGCGAGGAGCTGCGAGAGACGCTGACGCAAACCACG
GACGATGTGCGGTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4F8V4

Antitoxin

Source ID Structure
AlphaFold DB G0TQ86

References