Detailed information of TA system TA069548

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Rv0299-vapB/-
• Location: 363299..363870
• Replicon: chromosome
• Accession: NZ_CP018304
• Organism: Mycobacterium tuberculosis strain M0002959-6

Toxin (Protein)

• Gene name: Rv0299
• Uniprot ID: -
• Locus tag: BOK72_RS01605
• Protein ID: WP_003401560.1
• Coordinates: 363299..363601 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: O07227
• Locus tag: BOK72_RS01610
• Protein ID: WP_003401563.1
• Coordinates: 363649..363870 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BOK72_RS01585	358768..359571	-	804	WP_003401540.1	hypothetical protein	-
BOK72_RS01590	359581..360978	-	1398	WP_003401544.1	sulfatase	-
BOK72_RS01595	361157..362932	+	1776	WP_010886074.1	PE family protein	-
BOK72_RS01600	363075..363302	+	228	WP_003401555.1	ribbon-helix-helix protein, CopG family	-
BOK72_RS01605	363299..363601	+	303	WP_003401560.1	toxin-antitoxin system toxin	Toxin
BOK72_RS01610	363649..363870	+	222	WP_003401563.1	type II toxin-antitoxin system antitoxin VapB2	Antitoxin
BOK72_RS01615	363867..364292	+	426	WP_003401566.1	PIN domain nuclease	-
BOK72_RS01620	364427..365059	+	633	WP_003401571.1	TetR/AcrR family transcriptional regulator	-
BOK72_RS01625	365056..365964	+	909	WP_003900117.1	SDR family NAD(P)-dependent oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Ribbon-helix-helix protein, CopG (9-37)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 10442.16 Da
• Isoelectric Point: 4.9609

>T69548 WP_003401560.1 NZ_CP018304:363299-363601 [Mycobacterium tuberculosis]
MIAPGDIAPRRDSEHELYVAVLSNALHRAADTGRVITCPFIPGRVPEDLLAMVVAVEQPNGTLLPELVQWLHVAALGAPL
GNAGVAALREAASVVTALLC

Nucleotide

• Length: 303 bp

>T69548 NZ_CP018304:363299-363601 [Mycobacterium tuberculosis]
TTGATCGCTCCCGGCGACATCGCGCCGCGCCGCGACAGTGAACACGAGCTCTACGTCGCCGTCTTGTCCAACGCGCTCCA
TCGGGCCGCGGACACCGGACGGGTGATCACCTGCCCATTCATTCCGGGCCGGGTCCCCGAGGATCTCTTGGCGATGGTGG
TGGCGGTCGAGCAACCCAACGGCACGCTGCTGCCGGAACTCGTGCAGTGGCTTCATGTTGCCGCGCTCGGTGCGCCACTC
GGCAACGCGGGCGTGGCCGCCCTACGCGAGGCTGCCTCGGTCGTGACAGCTCTGCTCTGTTAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8088.31 Da
• Isoelectric Point: 10.7177

>AT69548 WP_003401563.1 NZ_CP018304:363649-363870 [Mycobacterium tuberculosis]
MSDVLIRDIPDDVLASLDAIAARLGLSRTEYIRRRLAQDAQTARVTVTAADLRRLRGAVAGLGDPELMRQAWR

Nucleotide

• Length: 222 bp

>AT69548 NZ_CP018304:363649-363870 [Mycobacterium tuberculosis]
ATGAGTGATGTACTGATTCGGGACATCCCCGACGACGTGTTAGCAAGCCTTGACGCGATCGCGGCACGCTTGGGCTTGTC
GCGGACCGAATACATCCGTCGGCGTTTAGCCCAGGATGCGCAGACGGCTCGCGTCACCGTGACAGCCGCGGATCTTCGAC
GCCTCAGGGGTGCGGTTGCCGGTCTGGGCGATCCCGAGCTTATGCGTCAGGCGTGGAGGTGA

Structures

Antitoxin

• PDB: 3H87
• AlphaFold DB: A0A829CG48