Detailed information of TA system TA069547

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Rv0298-Rv0299/-
• Location: 363075..363601
• Replicon: chromosome
• Accession: NZ_CP018304
• Organism: Mycobacterium tuberculosis strain M0002959-6

Toxin (Protein)

• Gene name: Rv0299
• Uniprot ID: -
• Locus tag: BOK72_RS01605
• Protein ID: WP_003401560.1
• Coordinates: 363299..363601 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: Rv0298
• Uniprot ID: P9WJ08
• Locus tag: BOK72_RS01600
• Protein ID: WP_003401555.1
• Coordinates: 363075..363302 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BOK72_RS01585	358768..359571	-	804	WP_003401540.1	hypothetical protein	-
BOK72_RS01590	359581..360978	-	1398	WP_003401544.1	sulfatase	-
BOK72_RS01595	361157..362932	+	1776	WP_010886074.1	PE family protein	-
BOK72_RS01600	363075..363302	+	228	WP_003401555.1	ribbon-helix-helix protein, CopG family	Antitoxin
BOK72_RS01605	363299..363601	+	303	WP_003401560.1	toxin-antitoxin system toxin	Toxin
BOK72_RS01610	363649..363870	+	222	WP_003401563.1	type II toxin-antitoxin system antitoxin VapB2	-
BOK72_RS01615	363867..364292	+	426	WP_003401566.1	PIN domain nuclease	-
BOK72_RS01620	364427..365059	+	633	WP_003401571.1	TetR/AcrR family transcriptional regulator	-
BOK72_RS01625	365056..365964	+	909	WP_003900117.1	SDR family NAD(P)-dependent oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Ribbon-helix-helix protein, CopG (6-41)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 10442.16 Da
• Isoelectric Point: 4.9609

>T69547 WP_003401560.1 NZ_CP018304:363299-363601 [Mycobacterium tuberculosis]
MIAPGDIAPRRDSEHELYVAVLSNALHRAADTGRVITCPFIPGRVPEDLLAMVVAVEQPNGTLLPELVQWLHVAALGAPL
GNAGVAALREAASVVTALLC

Nucleotide

• Length: 303 bp

>T69547 NZ_CP018304:363299-363601 [Mycobacterium tuberculosis]
TTGATCGCTCCCGGCGACATCGCGCCGCGCCGCGACAGTGAACACGAGCTCTACGTCGCCGTCTTGTCCAACGCGCTCCA
TCGGGCCGCGGACACCGGACGGGTGATCACCTGCCCATTCATTCCGGGCCGGGTCCCCGAGGATCTCTTGGCGATGGTGG
TGGCGGTCGAGCAACCCAACGGCACGCTGCTGCCGGAACTCGTGCAGTGGCTTCATGTTGCCGCGCTCGGTGCGCCACTC
GGCAACGCGGGCGTGGCCGCCCTACGCGAGGCTGCCTCGGTCGTGACAGCTCTGCTCTGTTAG

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8130.21 Da
• Isoelectric Point: 7.0084

>AT69547 WP_003401555.1 NZ_CP018304:363075-363302 [Mycobacterium tuberculosis]
MTKEKISVTVDAAVLAAIDADARAAGLNRSEMIEQALRNEHLRVALRDYTAKTVPALDIDAYAQRVYQANRAAGS

Nucleotide

• Length: 228 bp

>AT69547 NZ_CP018304:363075-363302 [Mycobacterium tuberculosis]
ATGACGAAAGAGAAGATCTCCGTGACGGTGGACGCGGCCGTCCTCGCGGCGATCGACGCGGACGCCAGGGCGGCGGGTTT
GAATCGGTCGGAAATGATTGAGCAGGCACTGCGCAACGAGCACCTGCGTGTCGCTCTGCGCGATTACACGGCTAAAACCG
TACCGGCGTTGGACATCGATGCCTACGCACAGCGGGTGTACCAGGCGAACCGGGCGGCCGGAAGTTGA

Structures

Antitoxin

• AlphaFold DB: A0A7U4F8T1