Detailed information of TA system TA069516

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2180157..2180860
• Replicon: chromosome
• Accession: NZ_CP018303
• Organism: Mycobacterium tuberculosis strain I0004241-1

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G0TLK7
• Locus tag: BOJ25_RS10490
• Protein ID: WP_003409778.1
• Coordinates: 2180157..2180486 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G0TLK8
• Locus tag: BOJ25_RS10495
• Protein ID: WP_003409780.1
• Coordinates: 2180483..2180860 (-)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BOJ25_RS10470	2176540..2177610	+	1071	WP_003899091.1	epoxide hydrolase EphB	-
BOJ25_RS10475	2177607..2178122	+	516	WP_003409718.1	flavin reductase family protein	-
BOJ25_RS10480	2178119..2179180	+	1062	WP_003899092.1	3,4-dihydroxy-2-butanone-4-phosphate synthase	-
BOJ25_RS10485	2179177..2179947	+	771	WP_003409775.1	SDR family oxidoreductase	-
BOJ25_RS10490	2180157..2180486	-	330	WP_003409778.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
BOJ25_RS10495	2180483..2180860	-	378	WP_003409780.1	type II toxin-antitoxin system antitoxin MazE5	Antitoxin
BOJ25_RS10500	2180857..2181447	-	591	WP_003409784.1	SEC-C domain-containing protein	-
BOJ25_RS10505	2181502..2182866	+	1365	WP_003903691.1	HNH endonuclease	-
BOJ25_RS10510	2183021..2183473	-	453	WP_003899095.1	lipoprotein	-
BOJ25_RS10515	2183537..2183938	+	402	WP_003409869.1	hypothetical protein	-
BOJ25_RS10520	2183931..2184113	-	183	WP_003409870.1	hypothetical protein	-
BOJ25_RS10525	2184227..2184577	-	351	WP_003899096.1	hypothetical protein	-
BOJ25_RS10530	2184588..2185490	-	903	WP_003899097.1	hypothetical protein	-
BOJ25_RS10535	2185511..2185702	-	192	WP_003409876.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-103)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 11787.82 Da
• Isoelectric Point: 8.5361

>T69516 WP_003409778.1 NZ_CP018303:c2180486-2180157 [Mycobacterium tuberculosis]
VTALPARGEVWWCEMAEIGRRPVVVLSRDAAIPRLRRALVAPCTTTIRGLASEVVLEPGSDPIPRRSAVNLDSVESVSVA
VLVNRLGRLADIRMRAICTALEVAVDCSR

Nucleotide

• Length: 330 bp

>T69516 NZ_CP018303:c2180486-2180157 [Mycobacterium tuberculosis]
GTGACCGCACTTCCGGCGCGCGGAGAGGTGTGGTGGTGTGAGATGGCTGAGATCGGTCGGCGACCAGTCGTCGTGCTGTC
GCGCGATGCCGCGATCCCTCGGCTGCGACGCGCACTTGTCGCGCCCTGCACCACGACCATCCGAGGGCTAGCCAGTGAGG
TTGTTCTTGAACCCGGTTCCGACCCGATCCCGCGCCGTTCCGCGGTGAATTTGGACTCAGTCGAAAGTGTCTCGGTCGCG
GTATTGGTGAATCGGCTTGGCCGCCTCGCCGACATCCGGATGCGCGCCATCTGCACGGCCCTCGAGGTCGCCGTCGATTG
CTCTCGATGA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13560.07 Da
• Isoelectric Point: 5.1519

>AT69516 WP_003409780.1 NZ_CP018303:c2180860-2180483 [Mycobacterium tuberculosis]
VKTARLQVTLRCAVDLINSSSDQCFARIEHVASDQADPRPGVWHSSGMNRIRLSTTVDAALLTSARDMRAGITDAALIDE
ALAALLARHRSAEVDASYAAYDKHPVDEPDEWGDLASWRRAAGDS

Nucleotide

• Length: 378 bp

>AT69516 NZ_CP018303:c2180860-2180483 [Mycobacterium tuberculosis]
GTGAAGACGGCCCGGTTGCAGGTGACGTTGCGCTGCGCGGTAGACCTCATCAATTCGTCCAGCGACCAATGTTTCGCCCG
TATCGAGCACGTTGCGTCCGATCAGGCGGATCCACGCCCCGGAGTGTGGCATAGTAGTGGCATGAATCGCATACGGCTGA
GCACTACTGTCGACGCCGCACTCTTGACAAGTGCGCGCGACATGCGGGCAGGAATCACTGACGCAGCCCTCATCGATGAG
GCCCTTGCGGCATTACTGGCTCGTCATCGATCGGCCGAGGTGGATGCCAGCTATGCGGCCTACGACAAGCACCCGGTCGA
TGAGCCCGACGAGTGGGGCGATCTAGCGTCGTGGCGGCGGGCGGCTGGAGACTCGTGA

Structures

Toxin

• AlphaFold DB: G0TLK7

Antitoxin

• AlphaFold DB: G0TLK8