Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VapB
Location 1761645..1762258 Replicon chromosome
Accession NZ_CP018303
Organism Mycobacterium tuberculosis strain I0004241-1

Toxin (Protein)


Gene name vapC Uniprot ID P64880
Locus tag BOJ25_RS08555 Protein ID WP_003407786.1
Coordinates 1761854..1762258 (+) Length 135 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A0E8UJ46
Locus tag BOJ25_RS08550 Protein ID WP_009935474.1
Coordinates 1761645..1761848 (+) Length 68 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BOJ25_RS08520 1757050..1757430 + 381 WP_003407768.1 fumarate reductase subunit C -
BOJ25_RS08525 1757427..1757804 + 378 WP_003407771.1 fumarate reductase subunit FrdD -
BOJ25_RS08530 1757872..1758480 + 609 WP_003407773.1 TetR/AcrR family transcriptional regulator -
BOJ25_RS08535 1758664..1759812 + 1149 Protein_1613 MMPL family transporter -
BOJ25_RS08540 1759822..1760268 + 447 WP_003407780.1 F420H(2)-dependent quinone reductase -
BOJ25_RS08545 1760303..1761592 + 1290 WP_003407781.1 threonine ammonia-lyase -
BOJ25_RS08550 1761645..1761848 + 204 WP_009935474.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
BOJ25_RS08555 1761854..1762258 + 405 WP_003407786.1 type II toxin-antitoxin system toxin ribonuclease VapC11 Toxin
BOJ25_RS08560 1762275..1764017 - 1743 WP_003407788.1 malto-oligosyltrehalose trehalohydrolase -
BOJ25_RS08565 1764010..1766307 - 2298 WP_003407790.1 malto-oligosyltrehalose synthase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-124)

Antitoxin

Bacterial antitoxin of type II TA system, VapB

(2-44)


Sequences

Toxin        


Download         Length: 135 a.a.        Molecular weight: 14650.76 Da        Isoelectric Point: 5.6587

>T69513 WP_003407786.1 NZ_CP018303:1761854-1762258 [Mycobacterium tuberculosis]
MILIDTSAWVEYFRATGSIAAVEVRRLLSEEAARIAMCEPIAMEILSGALDDNTHTTLERLVNGLPSLNVDDAIDFRAAA
GIYRAARRAGETVRSINDCLIAALAIRHGARIVHRDADFDVIARITNLQAASFR

Download         Length: 405 bp

>T69513 NZ_CP018303:1761854-1762258 [Mycobacterium tuberculosis]
ATGATCCTCATCGACACATCGGCCTGGGTGGAGTACTTCCGTGCCACCGGATCAATCGCCGCTGTCGAAGTACGCCGGCT
GCTGTCCGAAGAAGCAGCGCGAATCGCTATGTGTGAGCCCATTGCGATGGAAATCTTGAGTGGCGCGCTCGACGACAACA
CCCACACGACGCTAGAGCGGCTCGTGAATGGCTTGCCGTCGTTGAACGTTGATGACGCGATTGACTTTCGTGCTGCCGCG
GGTATCTATCGCGCCGCCCGGCGCGCCGGCGAAACGGTTCGAAGCATCAACGACTGCCTCATAGCGGCGCTCGCGATCCG
CCACGGTGCGCGTATCGTCCACCGTGACGCCGACTTTGATGTGATTGCCCGGATTACCAACCTGCAGGCCGCATCGTTTC
GGTGA

Antitoxin


Download         Length: 68 a.a.        Molecular weight: 7501.48 Da        Isoelectric Point: 4.3951

>AT69513 WP_009935474.1 NZ_CP018303:1761645-1761848 [Mycobacterium tuberculosis]
MSRTNIDIDDELAAEVMRRFGLTTKRAAVDLALRRLVGSPLSREFLLGLEGVGWEGDLDDLRSDRPD

Download         Length: 204 bp

>AT69513 NZ_CP018303:1761645-1761848 [Mycobacterium tuberculosis]
ATGTCGCGCACCAACATCGACATCGATGACGAACTTGCCGCCGAGGTCATGCGCAGGTTCGGTCTGACCACCAAGAGGGC
GGCGGTCGACCTTGCCCTACGACGGTTGGTCGGGTCGCCGTTGAGCCGTGAGTTTCTGCTCGGGCTGGAAGGCGTCGGCT
GGGAAGGCGACCTGGATGACTTGCGAAGCGATCGCCCAGACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6A7V

Antitoxin

Source ID Structure
AlphaFold DB A0A0E8UJ46

References