Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 3691270..3691944 Replicon chromosome
Accession NZ_CP018302
Organism Mycobacterium tuberculosis strain I0004000-1

Toxin (Protein)


Gene name vapC Uniprot ID P9WF52
Locus tag BOJ12_RS18105 Protein ID WP_003417282.1
Coordinates 3691270..3691698 (-) Length 143 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TI59
Locus tag BOJ12_RS18110 Protein ID WP_003417286.1
Coordinates 3691702..3691944 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BOJ12_RS18080 3687092..3687493 - 402 WP_003417264.1 cytidine deaminase -
BOJ12_RS18085 3687634..3688068 + 435 WP_003900017.1 succinate dehydrogenase, cytochrome b556 subunit -
BOJ12_RS18090 3688065..3688499 + 435 WP_003417269.1 succinate dehydrogenase hydrophobic membrane anchor subunit -
BOJ12_RS18095 3688628..3690400 + 1773 WP_003417273.1 succinate dehydrogenase flavoprotein subunit -
BOJ12_RS18100 3690400..3691191 + 792 WP_003417279.1 succinate dehydrogenase iron-sulfur subunit -
BOJ12_RS18105 3691270..3691698 - 429 WP_003417282.1 type II toxin-antitoxin system VapC family toxin Toxin
BOJ12_RS18110 3691702..3691944 - 243 WP_003417286.1 ribbon-helix-helix domain-containing protein Antitoxin
BOJ12_RS18115 3692066..3692680 - 615 WP_003417288.1 class I SAM-dependent methyltransferase -
BOJ12_RS18120 3692677..3693342 - 666 WP_003417290.1 molybdopterin converting factor subunit 1 -
BOJ12_RS18125 3693343..3693876 - 534 WP_003417293.1 cyclic pyranopterin monophosphate synthase MoaC -
BOJ12_RS18130 3693873..3694247 - 375 WP_003417295.1 4a-hydroxytetrahydrobiopterin dehydratase -
BOJ12_RS18145 3695702..3696766 - 1065 WP_003913037.1 GTP 3',8-cyclase MoaA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain toxin

(1-141)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 143 a.a.        Molecular weight: 15834.16 Da        Isoelectric Point: 8.5471

>T69481 WP_003417282.1 NZ_CP018302:c3691698-3691270 [Mycobacterium tuberculosis]
MRALLDVNVLLALLDRDHVDHERARAWITGQIERGWASCAITQNGFVRVISQPRYPSPISVAHAIDLLARATHTRYHEFW
SCTVSILDSKVIDRSRLHSPKQVTDAYLLALAVAHDGRFVTFDQSIALTAVPGATKQHLATL

Download         Length: 429 bp

>T69481 NZ_CP018302:c3691698-3691270 [Mycobacterium tuberculosis]
ATGCGAGCGCTGTTGGACGTCAACGTGCTACTGGCGCTGTTGGACCGCGATCACGTCGACCACGAGCGCGCCCGAGCGTG
GATCACCGGGCAGATCGAGCGGGGATGGGCTTCCTGTGCGATCACCCAAAACGGGTTCGTCCGCGTCATCAGCCAACCGC
GCTATCCCAGCCCAATCTCGGTAGCCCATGCCATCGACTTGTTGGCTCGTGCGACGCACACGCGCTATCACGAGTTCTGG
TCCTGCACCGTCAGCATTCTCGACTCAAAGGTCATCGATCGTTCCCGCCTGCACAGCCCCAAACAGGTCACCGACGCGTA
CTTGCTGGCCCTCGCGGTCGCTCATGATGGCCGATTTGTCACCTTCGATCAGTCGATTGCGTTGACTGCGGTTCCTGGCG
CAACCAAACAGCACCTGGCAACGCTGTAG

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 8849.86 Da        Isoelectric Point: 5.1007

>AT69481 WP_003417286.1 NZ_CP018302:c3691944-3691702 [Mycobacterium tuberculosis]
MRTTLSIDDDVLLAVKERARREKRTAGEILSDLARQALTNQNPQPAASQEDAFHGFEPLPHRGGAVSNALIDRLRDEEAV

Download         Length: 243 bp

>AT69481 NZ_CP018302:c3691944-3691702 [Mycobacterium tuberculosis]
ATGCGCACCACGTTGTCCATCGACGACGATGTATTGCTGGCCGTGAAGGAACGGGCACGCAGGGAGAAGCGCACCGCGGG
TGAAATCCTGTCGGATCTGGCCCGGCAAGCGTTAACCAACCAGAATCCCCAGCCTGCGGCGAGCCAGGAGGACGCCTTTC
ATGGCTTCGAACCCCTGCCTCATCGCGGTGGAGCGGTATCGAACGCGCTGATCGATCGGCTTCGTGACGAGGAGGCCGTG
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4FBL0

Antitoxin

Source ID Structure
AlphaFold DB G0TI59

References