Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 3094309..3094879 Replicon chromosome
Accession NZ_CP018302
Organism Mycobacterium tuberculosis strain I0004000-1

Toxin (Protein)


Gene name mazF Uniprot ID P71650
Locus tag BOJ12_RS15225 Protein ID WP_003414166.1
Coordinates 3094309..3094665 (-) Length 119 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P0CL61
Locus tag BOJ12_RS15230 Protein ID WP_003901465.1
Coordinates 3094649..3094879 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BOJ12_RS15205 3089761..3091449 - 1689 WP_003414155.1 alpha/beta hydrolase family protein -
BOJ12_RS15210 3091453..3091779 - 327 WP_003414157.1 hypothetical protein -
BOJ12_RS15215 3091952..3092539 + 588 WP_003914429.1 DUF3558 family protein -
BOJ12_RS15220 3092558..3094207 + 1650 WP_003899485.1 CocE/NonD family hydrolase -
BOJ12_RS15225 3094309..3094665 - 357 WP_003414166.1 type II toxin-antitoxin system toxin endoribonuclease MazF9 Toxin
BOJ12_RS15230 3094649..3094879 - 231 WP_003901465.1 antitoxin MazE Antitoxin
BOJ12_RS15235 3094922..3095965 - 1044 WP_003414172.1 DUF2293 domain-containing protein -
BOJ12_RS21665 3096156..3096431 + 276 WP_003911993.1 DUF1778 domain-containing protein -
BOJ12_RS21670 3096607..3096861 - 255 WP_003917684.1 hypothetical protein -
BOJ12_RS15255 3097009..3097413 + 405 WP_009938577.1 hypothetical protein -
BOJ12_RS15260 3097410..3097601 + 192 WP_003414184.1 hypothetical protein -
BOJ12_RS15265 3097665..3098954 + 1290 Protein_2882 transposase family protein -
BOJ12_RS15270 3099188..3099445 + 258 WP_003899489.1 hypothetical protein -
BOJ12_RS15275 3099550..3099861 + 312 WP_003414190.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-113)

Antitoxin

Ribbon-helix-helix protein, CopG

(3-29)


Sequences

Toxin        


Download         Length: 119 a.a.        Molecular weight: 12858.73 Da        Isoelectric Point: 9.1962

>T69475 WP_003414166.1 NZ_CP018302:c3094665-3094309 [Mycobacterium tuberculosis]
VMRRGEIWQVDLDPARGSEANNQRPAVVVSNDRANATATRLGRGVITVVPVTSNIAKVYPFQVLLSATTTGLQVDCKAQA
EQIRSIATERLLRPIGRVSAAELAQLDEALKLHLDLWS

Download         Length: 357 bp

>T69475 NZ_CP018302:c3094665-3094309 [Mycobacterium tuberculosis]
GTGATGCGCCGCGGTGAGATTTGGCAGGTCGATCTCGACCCCGCTCGAGGTAGCGAAGCGAACAACCAGCGCCCCGCCGT
CGTCGTCAGCAACGACCGGGCCAACGCGACCGCCACGCGTCTTGGGCGCGGCGTCATCACCGTCGTGCCGGTGACGAGCA
ACATCGCCAAGGTCTATCCGTTTCAGGTGTTGTTGTCGGCCACCACTACTGGTCTCCAGGTCGACTGCAAGGCGCAGGCC
GAGCAAATCAGATCGATTGCTACCGAGCGGTTGCTCCGGCCAATCGGCCGAGTTTCAGCCGCCGAACTTGCCCAGCTCGA
TGAGGCTTTGAAACTGCATCTCGACTTATGGTCGTAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8231.01 Da        Isoelectric Point: 4.1809

>AT69475 WP_003901465.1 NZ_CP018302:c3094879-3094649 [Mycobacterium tuberculosis]
VKLSVSLSDDDVAILDAYVKRAGLPSRSAGLQHAIRVLRYPTLEDDYANAWQEWSAAGDTDAWEQTVGDGVGDAPR

Download         Length: 231 bp

>AT69475 NZ_CP018302:c3094879-3094649 [Mycobacterium tuberculosis]
GTGAAGTTGAGCGTGAGCCTGTCCGATGACGACGTCGCGATCCTGGACGCATATGTGAAACGAGCGGGATTACCATCCCG
CTCCGCCGGCCTGCAGCATGCGATCCGCGTGCTTCGCTACCCCACGCTCGAAGATGACTATGCCAACGCATGGCAAGAAT
GGTCGGCAGCCGGCGACACGGACGCGTGGGAGCAGACCGTCGGCGACGGAGTCGGTGATGCGCCGCGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6L2A
PDB 5HJZ
PDB 6KYT
PDB 6L29
PDB 7DU5
PDB 6KYS

Antitoxin

Source ID Structure
PDB 7DU5
PDB 6KYT
AlphaFold DB A0A7U4FBW3

References