Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 2853869..2854506 Replicon chromosome
Accession NZ_CP018302
Organism Mycobacterium tuberculosis strain I0004000-1

Toxin (Protein)


Gene name vapC Uniprot ID P0DMV7
Locus tag BOJ12_RS13875 Protein ID WP_003413180.1
Coordinates 2853869..2854264 (-) Length 132 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ44
Locus tag BOJ12_RS13880 Protein ID WP_003413183.1
Coordinates 2854261..2854506 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BOJ12_RS13830 2849272..2850483 + 1212 WP_003900845.1 alpha/beta hydrolase family protein -
BOJ12_RS13840 2850610..2851269 + 660 WP_003900846.1 LppA family lipoprotein -
BOJ12_RS13845 2851266..2851928 + 663 WP_003900848.1 hypothetical protein -
BOJ12_RS13850 2851925..2852203 + 279 WP_003901422.1 type II toxin-antitoxin system VapB family antitoxin -
BOJ12_RS13855 2852296..2852709 + 414 WP_003413164.1 PIN domain nuclease -
BOJ12_RS13860 2852748..2853005 + 258 WP_003413167.1 ribbon-helix-helix domain-containing protein -
BOJ12_RS13865 2853002..2853379 + 378 WP_003413174.1 type II toxin-antitoxin system VapC family toxin -
BOJ12_RS13870 2853395..2853769 - 375 WP_003413177.1 hypothetical protein -
BOJ12_RS13875 2853869..2854264 - 396 WP_003413180.1 type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20 Toxin
BOJ12_RS13880 2854261..2854506 - 246 WP_003413183.1 type II toxin-antitoxin system antitoxin VapB20 Antitoxin
BOJ12_RS13890 2854917..2855336 - 420 WP_003413190.1 A24 family peptidase -
BOJ12_RS13895 2855348..2856157 - 810 WP_003413193.1 shikimate dehydrogenase -
BOJ12_RS13900 2856154..2857407 - 1254 WP_003413196.1 endolytic transglycosylase MltG -
BOJ12_RS13905 2857400..2857912 - 513 WP_003413197.1 Holliday junction resolvase RuvX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Endonuclease VapC20-like

(1-129)

Antitoxin

Ribbon-helix-helix protein, CopG

(11-49)


Sequences

Toxin        


Download         Length: 132 a.a.        Molecular weight: 14619.53 Da        Isoelectric Point: 6.9794

>T69470 WP_003413180.1 NZ_CP018302:c2854264-2853869 [Mycobacterium tuberculosis]
MIFVDTSFWAALGNAGDARHGTAKRLWASKPPVVMTSNHVLGETWTLLNRRCGHRAAVAAAAIRLSTVVRVEHVTADLEE
QAWEWLVRHDEREYSFVDATSFAVMRKKGIQNAYAFDGDFSAAGFVEVRPE

Download         Length: 396 bp

>T69470 NZ_CP018302:c2854264-2853869 [Mycobacterium tuberculosis]
ATGATCTTCGTCGACACGTCCTTCTGGGCTGCACTTGGCAACGCCGGCGACGCTCGGCACGGCACCGCGAAGAGGTTGTG
GGCCAGCAAACCGCCCGTGGTGATGACCTCCAACCATGTCCTGGGCGAGACCTGGACGCTGCTCAACCGGCGCTGCGGTC
ACCGCGCGGCGGTTGCCGCCGCTGCAATCCGCTTGAGCACCGTCGTTCGCGTCGAGCACGTAACAGCCGACCTGGAAGAG
CAGGCATGGGAATGGCTTGTACGCCACGACGAGCGAGAGTACTCGTTCGTCGATGCCACGAGCTTCGCGGTGATGCGAAA
GAAAGGCATCCAAAATGCGTATGCCTTCGATGGTGACTTCAGCGCGGCCGGATTTGTCGAGGTAAGACCCGAGTAG

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9098.33 Da        Isoelectric Point: 5.6007

>AT69470 WP_003413183.1 NZ_CP018302:c2854506-2854261 [Mycobacterium tuberculosis]
MLVAYICHVKRLQIYIDEDVDRALAVEARRRRTSKAALIREYVAEHLRQPGPDPVDAFVGSFVGEADLSASVDDVVYGKH
E

Download         Length: 246 bp

>AT69470 NZ_CP018302:c2854506-2854261 [Mycobacterium tuberculosis]
ATGCTAGTGGCGTATATTTGCCATGTGAAAAGGCTGCAGATCTACATCGACGAAGACGTTGACCGAGCGCTTGCTGTTGA
AGCGCGACGGCGGCGGACGTCGAAGGCGGCGCTGATCCGGGAGTACGTCGCTGAGCACCTCCGGCAGCCGGGCCCAGATC
CGGTCGATGCCTTCGTCGGATCGTTCGTGGGGGAAGCCGACTTGTCCGCGTCCGTCGACGACGTGGTTTACGGAAAGCAC
GAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5WZ4
PDB 5WZF

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4BW31

References