Detailed information of TA system TA069456

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2184914..2185617
• Replicon: chromosome
• Accession: NZ_CP018302
• Organism: Mycobacterium tuberculosis strain I0004000-1

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G0TLK7
• Locus tag: BOJ12_RS10505
• Protein ID: WP_003409778.1
• Coordinates: 2184914..2185243 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G0TLK8
• Locus tag: BOJ12_RS10510
• Protein ID: WP_003409780.1
• Coordinates: 2185240..2185617 (-)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BOJ12_RS10485	2181297..2182367	+	1071	WP_003899091.1	epoxide hydrolase EphB	-
BOJ12_RS10490	2182364..2182879	+	516	WP_003409718.1	flavin reductase family protein	-
BOJ12_RS10495	2182876..2183937	+	1062	WP_003899092.1	3,4-dihydroxy-2-butanone-4-phosphate synthase	-
BOJ12_RS10500	2183934..2184704	+	771	WP_003409775.1	SDR family oxidoreductase	-
BOJ12_RS10505	2184914..2185243	-	330	WP_003409778.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
BOJ12_RS10510	2185240..2185617	-	378	WP_003409780.1	type II toxin-antitoxin system antitoxin MazE5	Antitoxin
BOJ12_RS10515	2185614..2186204	-	591	WP_003409784.1	SEC-C domain-containing protein	-
BOJ12_RS10520	2186259..2187623	+	1365	WP_003906706.1	HNH endonuclease	-
BOJ12_RS10525	2187778..2188230	-	453	WP_003899095.1	lipoprotein	-
BOJ12_RS10530	2188294..2188695	+	402	WP_003409869.1	hypothetical protein	-
BOJ12_RS10535	2188688..2188870	-	183	WP_003409870.1	hypothetical protein	-
BOJ12_RS10540	2188984..2189334	-	351	WP_003899096.1	hypothetical protein	-
BOJ12_RS10545	2189345..2190247	-	903	WP_003899097.1	hypothetical protein	-
BOJ12_RS10550	2190268..2190459	-	192	WP_003409876.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-103)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 11787.82 Da
• Isoelectric Point: 8.5361

>T69456 WP_003409778.1 NZ_CP018302:c2185243-2184914 [Mycobacterium tuberculosis]
VTALPARGEVWWCEMAEIGRRPVVVLSRDAAIPRLRRALVAPCTTTIRGLASEVVLEPGSDPIPRRSAVNLDSVESVSVA
VLVNRLGRLADIRMRAICTALEVAVDCSR

Nucleotide

• Length: 330 bp

>T69456 NZ_CP018302:c2185243-2184914 [Mycobacterium tuberculosis]
GTGACCGCACTTCCGGCGCGCGGAGAGGTGTGGTGGTGTGAGATGGCTGAGATCGGTCGGCGACCAGTCGTCGTGCTGTC
GCGCGATGCCGCGATCCCTCGGCTGCGACGCGCACTTGTCGCGCCCTGCACCACGACCATCCGAGGGCTAGCCAGTGAGG
TTGTTCTTGAACCCGGTTCCGACCCGATCCCGCGCCGTTCCGCGGTGAATTTGGACTCAGTCGAAAGTGTCTCGGTCGCG
GTATTGGTGAATCGGCTTGGCCGCCTCGCCGACATCCGGATGCGCGCCATCTGCACGGCCCTCGAGGTCGCCGTCGATTG
CTCTCGATGA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13560.07 Da
• Isoelectric Point: 5.1519

>AT69456 WP_003409780.1 NZ_CP018302:c2185617-2185240 [Mycobacterium tuberculosis]
VKTARLQVTLRCAVDLINSSSDQCFARIEHVASDQADPRPGVWHSSGMNRIRLSTTVDAALLTSARDMRAGITDAALIDE
ALAALLARHRSAEVDASYAAYDKHPVDEPDEWGDLASWRRAAGDS

Nucleotide

• Length: 378 bp

>AT69456 NZ_CP018302:c2185617-2185240 [Mycobacterium tuberculosis]
GTGAAGACGGCCCGGTTGCAGGTGACGTTGCGCTGCGCGGTAGACCTCATCAATTCGTCCAGCGACCAATGTTTCGCCCG
TATCGAGCACGTTGCGTCCGATCAGGCGGATCCACGCCCCGGAGTGTGGCATAGTAGTGGCATGAATCGCATACGGCTGA
GCACTACTGTCGACGCCGCACTCTTGACAAGTGCGCGCGACATGCGGGCAGGAATCACTGACGCAGCCCTCATCGATGAG
GCCCTTGCGGCATTACTGGCTCGTCATCGATCGGCCGAGGTGGATGCCAGCTATGCGGCCTACGACAAGCACCCGGTCGA
TGAGCCCGACGAGTGGGGCGATCTAGCGTCGTGGCGGCGGGCGGCTGGAGACTCGTGA

Structures

Toxin

• AlphaFold DB: G0TLK7

Antitoxin

• AlphaFold DB: G0TLK8