Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 3685590..3686264 Replicon chromosome
Accession NZ_CP018301
Organism Mycobacterium tuberculosis strain I0002801-4

Toxin (Protein)


Gene name vapC Uniprot ID P9WF52
Locus tag BOJ61_RS18050 Protein ID WP_003417282.1
Coordinates 3685590..3686018 (-) Length 143 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TI59
Locus tag BOJ61_RS18055 Protein ID WP_003417286.1
Coordinates 3686022..3686264 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BOJ61_RS18025 3681412..3681813 - 402 WP_003417264.1 cytidine deaminase -
BOJ61_RS18030 3681954..3682388 + 435 WP_003900017.1 succinate dehydrogenase, cytochrome b556 subunit -
BOJ61_RS18035 3682385..3682819 + 435 WP_003417269.1 succinate dehydrogenase hydrophobic membrane anchor subunit -
BOJ61_RS18040 3682948..3684720 + 1773 WP_003417273.1 succinate dehydrogenase flavoprotein subunit -
BOJ61_RS18045 3684720..3685511 + 792 WP_003417279.1 succinate dehydrogenase iron-sulfur subunit -
BOJ61_RS18050 3685590..3686018 - 429 WP_003417282.1 type II toxin-antitoxin system VapC family toxin Toxin
BOJ61_RS18055 3686022..3686264 - 243 WP_003417286.1 ribbon-helix-helix domain-containing protein Antitoxin
BOJ61_RS18060 3686386..3687000 - 615 WP_003417288.1 class I SAM-dependent methyltransferase -
BOJ61_RS18065 3686997..3687662 - 666 WP_003417290.1 molybdopterin converting factor subunit 1 -
BOJ61_RS18070 3687663..3688196 - 534 WP_003417293.1 cyclic pyranopterin monophosphate synthase MoaC -
BOJ61_RS18075 3688193..3688567 - 375 WP_003417295.1 4a-hydroxytetrahydrobiopterin dehydratase -
BOJ61_RS18080 3688664..3689728 - 1065 WP_003913037.1 GTP 3',8-cyclase MoaA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain toxin

(1-141)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 143 a.a.        Molecular weight: 15834.16 Da        Isoelectric Point: 8.5471

>T69421 WP_003417282.1 NZ_CP018301:c3686018-3685590 [Mycobacterium tuberculosis]
MRALLDVNVLLALLDRDHVDHERARAWITGQIERGWASCAITQNGFVRVISQPRYPSPISVAHAIDLLARATHTRYHEFW
SCTVSILDSKVIDRSRLHSPKQVTDAYLLALAVAHDGRFVTFDQSIALTAVPGATKQHLATL

Download         Length: 429 bp

>T69421 NZ_CP018301:c3686018-3685590 [Mycobacterium tuberculosis]
ATGCGAGCGCTGTTGGACGTCAACGTGCTACTGGCGCTGTTGGACCGCGATCACGTCGACCACGAGCGCGCCCGAGCGTG
GATCACCGGGCAGATCGAGCGGGGATGGGCTTCCTGTGCGATCACCCAAAACGGGTTCGTCCGCGTCATCAGCCAACCGC
GCTATCCCAGCCCAATCTCGGTAGCCCATGCCATCGACTTGTTGGCTCGTGCGACGCACACGCGCTATCACGAGTTCTGG
TCCTGCACCGTCAGCATTCTCGACTCAAAGGTCATCGATCGTTCCCGCCTGCACAGCCCCAAACAGGTCACCGACGCGTA
CTTGCTGGCCCTCGCGGTCGCTCATGATGGCCGATTTGTCACCTTCGATCAGTCGATTGCGTTGACTGCGGTTCCTGGCG
CAACCAAACAGCACCTGGCAACGCTGTAG

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 8849.86 Da        Isoelectric Point: 5.1007

>AT69421 WP_003417286.1 NZ_CP018301:c3686264-3686022 [Mycobacterium tuberculosis]
MRTTLSIDDDVLLAVKERARREKRTAGEILSDLARQALTNQNPQPAASQEDAFHGFEPLPHRGGAVSNALIDRLRDEEAV

Download         Length: 243 bp

>AT69421 NZ_CP018301:c3686264-3686022 [Mycobacterium tuberculosis]
ATGCGCACCACGTTGTCCATCGACGACGATGTATTGCTGGCCGTGAAGGAACGGGCACGCAGGGAGAAGCGCACCGCGGG
TGAAATCCTGTCGGATCTGGCCCGGCAAGCGTTAACCAACCAGAATCCCCAGCCTGCGGCGAGCCAGGAGGACGCCTTTC
ATGGCTTCGAACCCCTGCCTCATCGCGGTGGAGCGGTATCGAACGCGCTGATCGATCGGCTTCGTGACGAGGAGGCCGTG
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4FBL0

Antitoxin

Source ID Structure
AlphaFold DB G0TI59

References