Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 3162949..3163636 Replicon chromosome
Accession NZ_CP018301
Organism Mycobacterium tuberculosis strain I0002801-4

Toxin (Protein)


Gene name vapC Uniprot ID P65044
Locus tag BOJ61_RS15565 Protein ID WP_003414624.1
Coordinates 3163193..3163636 (+) Length 148 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TFH0
Locus tag BOJ61_RS15560 Protein ID WP_003414620.1
Coordinates 3162949..3163206 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BOJ61_RS15540 3158269..3159123 - 855 WP_003899516.1 GNAT family N-acetyltransferase -
BOJ61_RS15545 3159179..3160342 - 1164 WP_003899517.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
BOJ61_RS15550 3160359..3161573 - 1215 WP_003899518.1 zinc metalloprotease Rip -
BOJ61_RS15555 3161581..3162822 - 1242 WP_003414613.1 1-deoxy-D-xylulose-5-phosphate reductoisomerase -
BOJ61_RS15560 3162949..3163206 + 258 WP_003414620.1 ribbon-helix-helix domain-containing protein Antitoxin
BOJ61_RS15565 3163193..3163636 + 444 WP_003414624.1 type II toxin-antitoxin system VapC family toxin Toxin
BOJ61_RS15570 3163716..3164378 + 663 WP_003414630.1 cell surface glycolipoprotein Mpt83 -
BOJ61_RS15575 3164474..3164665 + 192 WP_003414632.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
BOJ61_RS15580 3164997..3166745 + 1749 WP_003912869.1 cytochrome c biogenesis protein DipZ -
BOJ61_RS15585 3166841..3167422 + 582 WP_003414644.1 fasciclin domain-containing protein -
BOJ61_RS15590 3167522..3167788 + 267 WP_015456449.1 DUF2631 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain toxin

(1-142)

Antitoxin

Ribbon-helix-helix protein, CopG

(2-38)


Sequences

Toxin        


Download         Length: 148 a.a.        Molecular weight: 16595.72 Da        Isoelectric Point: 6.4890

>T69419 WP_003414624.1 NZ_CP018301:3163193-3163636 [Mycobacterium tuberculosis]
MLCVDVNVLVYAHRADLREHADYRGLLERLANDDEPLGLPDSVLAGFIRVVTNRRVFTEPTSPQDAWQAVDALLAAPAAM
RLRPGERHWMAFRQLASDVDANGNDIADAHLAAYALENNATWLSADRGFARFRRLRWRHPLDGQTHL

Download         Length: 444 bp

>T69419 NZ_CP018301:3163193-3163636 [Mycobacterium tuberculosis]
ATGCTGTGCGTTGATGTCAACGTGCTCGTTTACGCGCATCGGGCAGACCTACGGGAGCACGCGGACTATCGGGGTTTGCT
TGAGCGGCTGGCCAACGATGACGAGCCGCTGGGTCTACCAGATAGCGTGCTCGCCGGCTTCATCCGGGTGGTTACCAACC
GCCGCGTCTTCACCGAGCCGACGAGCCCACAGGACGCATGGCAGGCAGTCGACGCCCTACTCGCGGCACCCGCAGCCATG
CGACTTCGGCCTGGCGAGCGCCACTGGATGGCCTTTCGGCAGTTAGCGTCCGATGTTGATGCGAACGGCAACGACATTGC
GGACGCGCACCTGGCCGCCTACGCGCTAGAGAACAACGCAACCTGGTTGAGCGCCGACCGCGGCTTTGCCCGTTTCCGTC
GACTGCGCTGGCGTCATCCGTTGGACGGTCAGACCCATCTATAA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9013.22 Da        Isoelectric Point: 10.8344

>AT69419 WP_003414620.1 NZ_CP018301:3162949-3163206 [Mycobacterium tuberculosis]
VRTTIRIDDELYREVKAKAARSGRTVAAVLEDAVRRGLNPPKPQAAGRYRVQPSGKGGLRPGVDLSSNAALAEAMNDGVS
VDAVR

Download         Length: 258 bp

>AT69419 NZ_CP018301:3162949-3163206 [Mycobacterium tuberculosis]
GTGCGCACGACGATCCGTATCGATGACGAGCTGTACCGCGAGGTGAAAGCAAAGGCCGCTCGTTCCGGGCGTACCGTGGC
CGCGGTTCTTGAAGATGCGGTGCGGCGTGGTCTCAACCCGCCTAAGCCGCAGGCCGCCGGCCGTTATCGAGTCCAGCCGT
CGGGTAAGGGCGGCCTGCGGCCCGGTGTCGATCTATCGTCCAACGCCGCACTTGCCGAAGCGATGAACGACGGCGTGTCG
GTCGATGCTGTGCGTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7VWO

Antitoxin

Source ID Structure
PDB 7VWO
AlphaFold DB G0TFH0

References