Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 3090353..3090923 Replicon chromosome
Accession NZ_CP018301
Organism Mycobacterium tuberculosis strain I0002801-4

Toxin (Protein)


Gene name mazF Uniprot ID P71650
Locus tag BOJ61_RS15180 Protein ID WP_003414166.1
Coordinates 3090353..3090709 (-) Length 119 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P0CL61
Locus tag BOJ61_RS15185 Protein ID WP_003901465.1
Coordinates 3090693..3090923 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BOJ61_RS15160 3085805..3087493 - 1689 WP_010924579.1 alpha/beta hydrolase family protein -
BOJ61_RS15165 3087497..3087823 - 327 WP_003414157.1 hypothetical protein -
BOJ61_RS15170 3087996..3088583 + 588 WP_003914429.1 DUF3558 family protein -
BOJ61_RS15175 3088602..3090251 + 1650 WP_003899485.1 CocE/NonD family hydrolase -
BOJ61_RS15180 3090353..3090709 - 357 WP_003414166.1 type II toxin-antitoxin system toxin endoribonuclease MazF9 Toxin
BOJ61_RS15185 3090693..3090923 - 231 WP_003901465.1 antitoxin MazE Antitoxin
BOJ61_RS15190 3090966..3092009 - 1044 WP_003414172.1 DUF2293 domain-containing protein -
BOJ61_RS21625 3092200..3092475 + 276 WP_003911993.1 DUF1778 domain-containing protein -
BOJ61_RS21630 3092651..3092905 - 255 WP_003917684.1 hypothetical protein -
BOJ61_RS15210 3093053..3093457 + 405 WP_009938577.1 hypothetical protein -
BOJ61_RS15215 3093454..3093645 + 192 WP_003414184.1 hypothetical protein -
BOJ61_RS15220 3093709..3094998 + 1290 Protein_2878 transposase family protein -
BOJ61_RS15225 3095232..3095489 + 258 WP_003899489.1 hypothetical protein -
BOJ61_RS15230 3095594..3095905 + 312 WP_003414190.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-113)

Antitoxin

Ribbon-helix-helix protein, CopG

(3-29)


Sequences

Toxin        


Download         Length: 119 a.a.        Molecular weight: 12858.73 Da        Isoelectric Point: 9.1962

>T69415 WP_003414166.1 NZ_CP018301:c3090709-3090353 [Mycobacterium tuberculosis]
VMRRGEIWQVDLDPARGSEANNQRPAVVVSNDRANATATRLGRGVITVVPVTSNIAKVYPFQVLLSATTTGLQVDCKAQA
EQIRSIATERLLRPIGRVSAAELAQLDEALKLHLDLWS

Download         Length: 357 bp

>T69415 NZ_CP018301:c3090709-3090353 [Mycobacterium tuberculosis]
GTGATGCGCCGCGGTGAGATTTGGCAGGTCGATCTCGACCCCGCTCGAGGTAGCGAAGCGAACAACCAGCGCCCCGCCGT
CGTCGTCAGCAACGACCGGGCCAACGCGACCGCCACGCGTCTTGGGCGCGGCGTCATCACCGTCGTGCCGGTGACGAGCA
ACATCGCCAAGGTCTATCCGTTTCAGGTGTTGTTGTCGGCCACCACTACTGGTCTCCAGGTCGACTGCAAGGCGCAGGCC
GAGCAAATCAGATCGATTGCTACCGAGCGGTTGCTCCGGCCAATCGGCCGAGTTTCAGCCGCCGAACTTGCCCAGCTCGA
TGAGGCTTTGAAACTGCATCTCGACTTATGGTCGTAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8231.01 Da        Isoelectric Point: 4.1809

>AT69415 WP_003901465.1 NZ_CP018301:c3090923-3090693 [Mycobacterium tuberculosis]
VKLSVSLSDDDVAILDAYVKRAGLPSRSAGLQHAIRVLRYPTLEDDYANAWQEWSAAGDTDAWEQTVGDGVGDAPR

Download         Length: 231 bp

>AT69415 NZ_CP018301:c3090923-3090693 [Mycobacterium tuberculosis]
GTGAAGTTGAGCGTGAGCCTGTCCGATGACGACGTCGCGATCCTGGACGCATATGTGAAACGAGCGGGATTACCATCCCG
CTCCGCCGGCCTGCAGCATGCGATCCGCGTGCTTCGCTACCCCACGCTCGAAGATGACTATGCCAACGCATGGCAAGAAT
GGTCGGCAGCCGGCGACACGGACGCGTGGGAGCAGACCGTCGGCGACGGAGTCGGTGATGCGCCGCGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6L2A
PDB 5HJZ
PDB 6KYT
PDB 6L29
PDB 7DU5
PDB 6KYS

Antitoxin

Source ID Structure
PDB 7DU5
PDB 6KYT
AlphaFold DB A0A7U4FBW3

References