Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 2851272..2851909 Replicon chromosome
Accession NZ_CP018301
Organism Mycobacterium tuberculosis strain I0002801-4

Toxin (Protein)


Gene name vapC Uniprot ID P0DMV7
Locus tag BOJ61_RS13845 Protein ID WP_003413180.1
Coordinates 2851272..2851667 (-) Length 132 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ44
Locus tag BOJ61_RS13850 Protein ID WP_003413183.1
Coordinates 2851664..2851909 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BOJ61_RS13800 2846675..2847886 + 1212 WP_003900845.1 alpha/beta hydrolase family protein -
BOJ61_RS13810 2848013..2848672 + 660 WP_003900846.1 LppA family lipoprotein -
BOJ61_RS13815 2848669..2849331 + 663 WP_003900848.1 hypothetical protein -
BOJ61_RS13820 2849328..2849606 + 279 WP_003901422.1 type II toxin-antitoxin system VapB family antitoxin -
BOJ61_RS13825 2849699..2850112 + 414 WP_003413164.1 PIN domain nuclease -
BOJ61_RS13830 2850151..2850408 + 258 WP_003413167.1 ribbon-helix-helix domain-containing protein -
BOJ61_RS13835 2850405..2850782 + 378 WP_003413174.1 type II toxin-antitoxin system VapC family toxin -
BOJ61_RS13840 2850798..2851172 - 375 WP_003413177.1 hypothetical protein -
BOJ61_RS13845 2851272..2851667 - 396 WP_003413180.1 type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20 Toxin
BOJ61_RS13850 2851664..2851909 - 246 WP_003413183.1 type II toxin-antitoxin system antitoxin VapB20 Antitoxin
BOJ61_RS13860 2852320..2852739 - 420 WP_003413190.1 A24 family peptidase -
BOJ61_RS13865 2852751..2853560 - 810 WP_003413193.1 shikimate dehydrogenase -
BOJ61_RS13870 2853557..2854813 - 1257 WP_014585306.1 endolytic transglycosylase MltG -
BOJ61_RS13875 2854806..2855318 - 513 WP_003413197.1 Holliday junction resolvase RuvX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Endonuclease VapC20-like

(1-129)

Antitoxin

Ribbon-helix-helix protein, CopG

(11-49)


Sequences

Toxin        


Download         Length: 132 a.a.        Molecular weight: 14619.53 Da        Isoelectric Point: 6.9794

>T69410 WP_003413180.1 NZ_CP018301:c2851667-2851272 [Mycobacterium tuberculosis]
MIFVDTSFWAALGNAGDARHGTAKRLWASKPPVVMTSNHVLGETWTLLNRRCGHRAAVAAAAIRLSTVVRVEHVTADLEE
QAWEWLVRHDEREYSFVDATSFAVMRKKGIQNAYAFDGDFSAAGFVEVRPE

Download         Length: 396 bp

>T69410 NZ_CP018301:c2851667-2851272 [Mycobacterium tuberculosis]
ATGATCTTCGTCGACACGTCCTTCTGGGCTGCACTTGGCAACGCCGGCGACGCTCGGCACGGCACCGCGAAGAGGTTGTG
GGCCAGCAAACCGCCCGTGGTGATGACCTCCAACCATGTCCTGGGCGAGACCTGGACGCTGCTCAACCGGCGCTGCGGTC
ACCGCGCGGCGGTTGCCGCCGCTGCAATCCGCTTGAGCACCGTCGTTCGCGTCGAGCACGTAACAGCCGACCTGGAAGAG
CAGGCATGGGAATGGCTTGTACGCCACGACGAGCGAGAGTACTCGTTCGTCGATGCCACGAGCTTCGCGGTGATGCGAAA
GAAAGGCATCCAAAATGCGTATGCCTTCGATGGTGACTTCAGCGCGGCCGGATTTGTCGAGGTAAGACCCGAGTAG

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9098.33 Da        Isoelectric Point: 5.6007

>AT69410 WP_003413183.1 NZ_CP018301:c2851909-2851664 [Mycobacterium tuberculosis]
MLVAYICHVKRLQIYIDEDVDRALAVEARRRRTSKAALIREYVAEHLRQPGPDPVDAFVGSFVGEADLSASVDDVVYGKH
E

Download         Length: 246 bp

>AT69410 NZ_CP018301:c2851909-2851664 [Mycobacterium tuberculosis]
ATGCTAGTGGCGTATATTTGCCATGTGAAAAGGCTGCAGATCTACATCGACGAAGACGTTGACCGAGCGCTTGCTGTTGA
AGCGCGACGGCGGCGGACGTCGAAGGCGGCGCTGATCCGGGAGTACGTCGCTGAGCACCTCCGGCAGCCGGGCCCAGATC
CGGTCGATGCCTTCGTCGGATCGTTCGTGGGGGAAGCCGACTTGTCCGCGTCCGTCGACGACGTGGTTTACGGAAAGCAC
GAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5WZ4
PDB 5WZF

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4BW31

References