Detailed information of TA system TA069401

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 2243993..2244619
• Replicon: chromosome
• Accession: NZ_CP018301
• Organism: Mycobacterium tuberculosis strain I0002801-4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P64926
• Locus tag: BOJ61_RS10865
• Protein ID: WP_003410075.1
• Coordinates: 2244221..2244619 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A806JLL8
• Locus tag: BOJ61_RS10860
• Protein ID: WP_003911750.1
• Coordinates: 2243993..2244220 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BOJ61_RS10850	2242032..2242376	-	345	WP_003410065.1	ferredoxin family protein	-
BOJ61_RS10855	2242565..2243734	-	1170	WP_003899126.1	ATP-binding protein	-
BOJ61_RS10860	2243993..2244220	+	228	WP_003911750.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
BOJ61_RS10865	2244221..2244619	+	399	WP_003410075.1	PIN domain nuclease	Toxin
BOJ61_RS10870	2244802..2245233	-	432	WP_003410078.1	MarR family winged helix-turn-helix transcriptional regulator	-
BOJ61_RS10875	2245334..2245768	+	435	WP_003914358.1	DUF1398 domain-containing protein	-
BOJ61_RS10890	2246205..2246384	-	180	Protein_2060	hypothetical protein	-
BOJ61_RS10895	2246445..2247092	+	648	WP_071854215.1	transposase	-
BOJ61_RS10900	2247046..2247636	+	591	WP_003899131.1	IS110 family transposase	-
BOJ61_RS10905	2247764..2249020	-	1257	WP_003410095.1	HNH endonuclease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-120)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (2-43)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14731.02 Da
• Isoelectric Point: 6.7067

>T69401 WP_003410075.1 NZ_CP018301:2244221-2244619 [Mycobacterium tuberculosis]
MIVDTSVWIAYLSTSESLASRWLADRIAADSTVIVPEVVMMELLIGKTDEDTAALRRRLLQRFAIEPLAPVRDAEDAAAI
HRRCRRGGDTVRSLIDCQVAAMALRIGVAVAHRDRDYEAIRTHCGLRTEPLF

Nucleotide

• Length: 399 bp

>T69401 NZ_CP018301:2244221-2244619 [Mycobacterium tuberculosis]
ATGATCGTCGACACCTCGGTCTGGATCGCATATCTCTCCACGTCAGAGTCGTTGGCCAGTCGCTGGCTAGCCGATCGCAT
TGCCGCTGACTCGACGGTGATCGTGCCCGAGGTGGTGATGATGGAGCTGCTGATCGGTAAGACCGATGAGGACACCGCCG
CACTGCGCCGACGGCTCCTGCAGCGATTCGCTATCGAACCGCTGGCCCCGGTCCGCGACGCGGAAGATGCCGCCGCCATT
CACCGGCGCTGTCGTCGCGGCGGCGACACCGTACGCAGCCTGATCGATTGCCAGGTGGCCGCGATGGCGTTGCGGATCGG
GGTCGCCGTGGCGCATCGTGATCGCGACTACGAGGCGATCCGCACACATTGCGGACTACGCACCGAGCCGTTGTTCTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8346.16 Da
• Isoelectric Point: 4.1621

>AT69401 WP_003911750.1 NZ_CP018301:2243993-2244220 [Mycobacterium tuberculosis]
VSRTNIEIDDELVAAAQRMYRLDSKRSAVDLALRRLVGEPLGRDEALALQGSGFDFSNDEIESFSDTDRKLADES

Nucleotide

• Length: 228 bp

>AT69401 NZ_CP018301:2243993-2244220 [Mycobacterium tuberculosis]
GTGTCACGGACCAACATCGAGATCGACGACGAACTCGTGGCCGCCGCACAGCGGATGTACCGACTCGATTCCAAGCGAAG
TGCCGTCGACCTCGCGCTGCGCCGGCTCGTGGGTGAACCGTTGGGCCGCGATGAGGCTTTGGCGCTGCAGGGCAGCGGTT
TCGACTTCAGCAACGATGAGATCGAATCGTTCTCGGATACGGACCGCAAGCTCGCCGACGAGTCGTAG

Structures

Toxin

• PDB: 4CHG

Antitoxin

• AlphaFold DB: A0A806JLL8