Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2180591..2181294 Replicon chromosome
Accession NZ_CP018301
Organism Mycobacterium tuberculosis strain I0002801-4

Toxin (Protein)


Gene name mazF Uniprot ID G0TLK7
Locus tag BOJ61_RS10475 Protein ID WP_003409778.1
Coordinates 2180591..2180920 (-) Length 110 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G0TLK8
Locus tag BOJ61_RS10480 Protein ID WP_003409780.1
Coordinates 2180917..2181294 (-) Length 126 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BOJ61_RS10455 2176974..2178044 + 1071 WP_003899091.1 epoxide hydrolase EphB -
BOJ61_RS10460 2178041..2178556 + 516 WP_003409718.1 flavin reductase family protein -
BOJ61_RS10465 2178553..2179614 + 1062 WP_003899092.1 3,4-dihydroxy-2-butanone-4-phosphate synthase -
BOJ61_RS10470 2179611..2180381 + 771 WP_003409775.1 SDR family oxidoreductase -
BOJ61_RS10475 2180591..2180920 - 330 WP_003409778.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
BOJ61_RS10480 2180917..2181294 - 378 WP_003409780.1 type II toxin-antitoxin system antitoxin MazE5 Antitoxin
BOJ61_RS10485 2181291..2181881 - 591 WP_003409784.1 SEC-C domain-containing protein -
BOJ61_RS10490 2181936..2183300 + 1365 WP_070891469.1 HNH endonuclease -
BOJ61_RS10495 2183455..2183907 - 453 WP_003899095.1 lipoprotein -
BOJ61_RS10500 2183971..2184372 + 402 WP_003409869.1 hypothetical protein -
BOJ61_RS10505 2184365..2184547 - 183 WP_003409870.1 hypothetical protein -
BOJ61_RS10510 2184661..2185011 - 351 WP_003899096.1 hypothetical protein -
BOJ61_RS10515 2185022..2185924 - 903 WP_003899097.1 hypothetical protein -
BOJ61_RS10520 2185945..2186136 - 192 WP_003409876.1 hypothetical protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(7-103)

Antitoxin


No domain identified.



Sequences


Toxin        


Download         Length: 110 a.a.        Molecular weight: 11787.82 Da        Isoelectric Point: 8.5361

>T69395 WP_003409778.1 NZ_CP018301:c2180920-2180591 [Mycobacterium tuberculosis]
VTALPARGEVWWCEMAEIGRRPVVVLSRDAAIPRLRRALVAPCTTTIRGLASEVVLEPGSDPIPRRSAVNLDSVESVSVA
VLVNRLGRLADIRMRAICTALEVAVDCSR

Download         Length: 330 bp

>T69395 NZ_CP018301:c2180920-2180591 [Mycobacterium tuberculosis]
GTGACCGCACTTCCGGCGCGCGGAGAGGTGTGGTGGTGTGAGATGGCTGAGATCGGTCGGCGACCAGTCGTCGTGCTGTC
GCGCGATGCCGCGATCCCTCGGCTGCGACGCGCACTTGTCGCGCCCTGCACCACGACCATCCGAGGGCTAGCCAGTGAGG
TTGTTCTTGAACCCGGTTCCGACCCGATCCCGCGCCGTTCCGCGGTGAATTTGGACTCAGTCGAAAGTGTCTCGGTCGCG
GTATTGGTGAATCGGCTTGGCCGCCTCGCCGACATCCGGATGCGCGCCATCTGCACGGCCCTCGAGGTCGCCGTCGATTG
CTCTCGATGA

Antitoxin


Download         Length: 126 a.a.        Molecular weight: 13560.07 Da        Isoelectric Point: 5.1519

>AT69395 WP_003409780.1 NZ_CP018301:c2181294-2180917 [Mycobacterium tuberculosis]
VKTARLQVTLRCAVDLINSSSDQCFARIEHVASDQADPRPGVWHSSGMNRIRLSTTVDAALLTSARDMRAGITDAALIDE
ALAALLARHRSAEVDASYAAYDKHPVDEPDEWGDLASWRRAAGDS

Download         Length: 378 bp

>AT69395 NZ_CP018301:c2181294-2180917 [Mycobacterium tuberculosis]
GTGAAGACGGCCCGGTTGCAGGTGACGTTGCGCTGCGCGGTAGACCTCATCAATTCGTCCAGCGACCAATGTTTCGCCCG
TATCGAGCACGTTGCGTCCGATCAGGCGGATCCACGCCCCGGAGTGTGGCATAGTAGTGGCATGAATCGCATACGGCTGA
GCACTACTGTCGACGCCGCACTCTTGACAAGTGCGCGCGACATGCGGGCAGGAATCACTGACGCAGCCCTCATCGATGAG
GCCCTTGCGGCATTACTGGCTCGTCATCGATCGGCCGAGGTGGATGCCAGCTATGCGGCCTACGACAAGCACCCGGTCGA
TGAGCCCGACGAGTGGGGCGATCTAGCGTCGTGGCGGCGGGCGGCTGGAGACTCGTGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB G0TLK7


Antitoxin

Source ID Structure
AlphaFold DB G0TLK8

References