Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 1934778..1935391 Replicon chromosome
Accession NZ_CP018301
Organism Mycobacterium tuberculosis strain I0002801-4

Toxin (Protein)


Gene name vapC Uniprot ID P9WFA2
Locus tag BOJ61_RS09295 Protein ID WP_003408465.1
Coordinates 1934778..1935167 (-) Length 130 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ52
Locus tag BOJ61_RS09300 Protein ID WP_003408469.1
Coordinates 1935164..1935391 (-) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BOJ61_RS09260 1930407..1931321 + 915 WP_003898985.1 3-hydroxyacyl-CoA dehydrogenase family protein -
BOJ61_RS09265 1931324..1932154 + 831 WP_003408448.1 cyclase family protein -
BOJ61_RS09270 1932154..1932504 + 351 WP_003898986.1 cupin domain-containing protein -
BOJ61_RS09275 1932557..1933375 + 819 WP_003408456.1 3-keto-5-aminohexanoate cleavage protein -
BOJ61_RS09280 1933389..1934168 + 780 WP_003408460.1 IclR family transcriptional regulator -
BOJ61_RS09295 1934778..1935167 - 390 WP_003408465.1 PIN domain-containing protein Toxin
BOJ61_RS09300 1935164..1935391 - 228 WP_003408469.1 antitoxin Antitoxin
BOJ61_RS09305 1935609..1937093 + 1485 WP_003408473.1 carboxylase -
BOJ61_RS09310 1937090..1938337 + 1248 WP_003408476.1 serine hydrolase -
BOJ61_RS09315 1938380..1938799 - 420 WP_003408483.1 hypothetical protein -
BOJ61_RS09320 1938789..1939498 - 710 Protein_1764 winged helix-turn-helix transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-116)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 130 a.a.        Molecular weight: 13817.98 Da        Isoelectric Point: 7.0612

>T69393 WP_003408465.1 NZ_CP018301:c1935167-1934778 [Mycobacterium tuberculosis]
VIVLDASAAVELMLTTPAGAAVARRLRGETVHAPAHFDVEVIGAIRQAVVRQLISDHEGLVVVVNFLSLPVRRWPLKPFT
QRAYQLRSTHTVADGAYVALAEGLGVPLITCDGRLAQSHGHNAEIELVA

Download         Length: 390 bp

>T69393 NZ_CP018301:c1935167-1934778 [Mycobacterium tuberculosis]
GTGATCGTGTTGGACGCCTCGGCGGCCGTCGAGCTCATGCTCACGACGCCGGCGGGTGCGGCGGTCGCCCGGCGCTTGCG
GGGCGAAACCGTGCACGCCCCTGCACATTTCGATGTCGAGGTGATCGGGGCGATACGTCAAGCGGTCGTGCGTCAGCTGA
TCAGCGATCATGAAGGACTCGTCGTGGTTGTCAACTTCCTTAGCCTGCCCGTTCGGCGTTGGCCGTTAAAGCCATTCACC
CAGCGTGCCTACCAACTTCGGTCCACTCATACCGTGGCCGATGGAGCCTACGTCGCCCTCGCCGAGGGCCTCGGCGTACC
GCTTATCACTTGCGACGGGCGACTTGCCCAATCCCATGGCCATAATGCGGAGATCGAGCTTGTCGCCTGA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8226.39 Da        Isoelectric Point: 4.3313

>AT69393 WP_003408469.1 NZ_CP018301:c1935391-1935164 [Mycobacterium tuberculosis]
MSAMVQIRNVPDELLHELKARAAAQRMSLSDFLLARLAEIAEEPALDDVLDRLAALPRRDLGASAAELVDEARSE

Download         Length: 228 bp

>AT69393 NZ_CP018301:c1935391-1935164 [Mycobacterium tuberculosis]
ATGTCCGCCATGGTTCAGATCCGCAACGTTCCCGACGAGCTTCTCCACGAGCTGAAGGCCCGCGCAGCGGCGCAACGGAT
GAGCCTGAGCGATTTCCTCCTAGCACGGCTTGCCGAGATCGCGGAAGAACCGGCCCTCGACGACGTTCTCGACCGTCTAG
CGGCACTGCCGCGCCGGGATCTCGGCGCGAGCGCAGCCGAGCTTGTTGACGAGGCTCGATCTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BUI9

Antitoxin

Source ID Structure
PDB 7E4J
AlphaFold DB A0A7U4FAN9

References