Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) vapBC/AbrB(antitoxin)
Location 698377..699023 Replicon chromosome
Accession NZ_CP018301
Organism Mycobacterium tuberculosis strain I0002801-4

Toxin (Protein)


Gene name vapC Uniprot ID G0TQ88
Locus tag BOJ61_RS03195 Protein ID WP_003403137.1
Coordinates 698377..698790 (-) Length 138 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TQ89
Locus tag BOJ61_RS03200 Protein ID WP_003403139.1
Coordinates 698787..699023 (-) Length 79 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BOJ61_RS03175 694460..696010 + 1551 WP_003403119.1 MCE family protein -
BOJ61_RS03180 696062..696454 - 393 WP_003403122.1 type II toxin-antitoxin system toxin ribonuclease C4 -
BOJ61_RS03185 696451..696708 - 258 WP_003403125.1 type II toxin-antitoxin system antitoxin VapB4 -
BOJ61_RS03190 696891..698126 - 1236 WP_003403128.1 ATP-binding protein -
BOJ61_RS03195 698377..698790 - 414 WP_003403137.1 type II toxin-antitoxin system VapC family toxin Toxin
BOJ61_RS03200 698787..699023 - 237 WP_003403139.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
BOJ61_RS03205 699127..699633 - 507 WP_003900973.1 two component system sensor kinase HK1 -
BOJ61_RS03210 699747..700277 - 531 WP_003917320.1 two component system sensor kinase HK2 -
BOJ61_RS03215 700261..701022 - 762 WP_003403148.1 two-component system response regulator TcrA -
BOJ61_RS03220 701079..701390 + 312 WP_003403164.1 hypothetical protein -
BOJ61_RS03225 701462..702412 + 951 WP_003900184.1 DUF1259 domain-containing protein -
BOJ61_RS03230 702653..703237 + 585 WP_003403171.1 IS607-like element IS1536 family transposase -
BOJ61_RS03235 703239..703949 + 711 Protein_628 transposase -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(7-126)

Antitoxin

(3-43)


Sequences


Toxin        


Download         Length: 138 a.a.        Molecular weight: 14786.14 Da        Isoelectric Point: 8.1405

>T69372 WP_003403137.1 NZ_CP018301:c698790-698377 [Mycobacterium tuberculosis]
VKPPLAVDTSVAIPLLVRTHTAHAAVVAWWAHREAALCGHALAETYSVLTRLPRDLRLAPMDAARLLTERFAAPLLLSSR
TTEHLPRVLAQFEITGGAVYDALVALAAAEHRAELATRDARAKDTYEKIGVHVVVAA

Download         Length: 414 bp

>T69372 NZ_CP018301:c698790-698377 [Mycobacterium tuberculosis]
GTGAAACCGCCGCTGGCAGTGGACACCAGCGTCGCGATTCCGTTGCTGGTACGGACCCATACCGCGCATGCGGCGGTTGT
CGCCTGGTGGGCACATCGGGAGGCCGCGCTGTGCGGGCACGCCCTGGCCGAAACCTACTCCGTGCTGACCCGCCTGCCGC
GTGACCTACGACTGGCACCGATGGACGCCGCGCGGCTTCTTACCGAACGCTTTGCGGCACCGCTGTTGTTGAGTTCCCGC
ACCACTGAACATCTCCCACGCGTGCTGGCCCAATTCGAGATCACTGGTGGCGCGGTCTATGACGCTCTCGTGGCCCTTGC
CGCCGCCGAGCACCGGGCTGAACTCGCCACACGCGATGCTCGAGCCAAGGACACTTACGAAAAGATCGGCGTCCACGTCG
TTGTCGCCGCGTGA

Antitoxin


Download         Length: 79 a.a.        Molecular weight: 8212.41 Da        Isoelectric Point: 5.0351

>AT69372 WP_003403139.1 NZ_CP018301:c699023-698787 [Mycobacterium tuberculosis]
MKAVVDAAGRIVVPKPLREALGLQPGSTVEISRYGAGLHLIPTGRTARLEEENGVLVATGETTIDDEVVFGLIDSGRK

Download         Length: 237 bp

>AT69372 NZ_CP018301:c699023-698787 [Mycobacterium tuberculosis]
ATGAAGGCTGTTGTGGATGCGGCTGGCCGCATCGTCGTACCCAAACCGCTGCGCGAAGCCTTGGGGCTGCAACCGGGGTC
CACCGTGGAAATATCCCGCTATGGCGCTGGTCTCCACCTGATTCCCACCGGACGAACAGCGCGTCTCGAAGAGGAGAATG
GCGTACTGGTGGCCACCGGTGAGACCACGATCGACGATGAGGTCGTGTTCGGCCTTATCGATAGCGGCCGGAAGTGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB G0TQ88


Antitoxin

Source ID Structure
AlphaFold DB G0TQ89

References