Detailed information of TA system TA069325

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1235383..1235951
• Replicon: chromosome
• Accession: NZ_CP018300
• Organism: Mycobacterium tuberculosis strain I0002353-6

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TH08
• Locus tag: BOM19_RS06095
• Protein ID: WP_003405865.1
• Coordinates: 1235577..1235951 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TH07
• Locus tag: BOM19_RS06090
• Protein ID: WP_003405863.1
• Coordinates: 1235383..1235580 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BOM19_RS06070	1231424..1232062	-	639	WP_003898730.1	lipid droplet-associated protein	-
BOM19_RS06075	1232152..1233159	+	1008	WP_003405853.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
BOM19_RS06080	1233176..1234159	-	984	WP_003898731.1	hypothetical protein	-
BOM19_RS06085	1234222..1235295	+	1074	WP_003898732.1	redox-regulated ATPase YchF	-
BOM19_RS06090	1235383..1235580	+	198	WP_003405863.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
BOM19_RS06095	1235577..1235951	+	375	WP_003405865.1	type II toxin-antitoxin system VapC family toxin	Toxin
BOM19_RS06100	1236154..1236852	+	699	WP_003898733.1	hypothetical protein	-
BOM19_RS06105	1236970..1237155	+	186	WP_003901093.1	hypothetical protein	-
BOM19_RS06110	1237082..1237357	-	276	WP_003405867.1	hypothetical protein	-
BOM19_RS06115	1237600..1237923	+	324	WP_003405871.1	antibiotic biosynthesis monooxygenase	-
BOM19_RS06120	1237938..1238648	-	711	WP_003911399.1	hypothetical protein	-
BOM19_RS21905	1238670..1238879	+	210	WP_003911400.1	hypothetical protein	-
BOM19_RS06130	1238831..1239538	-	708	Protein_1161	adenylate/guanylate cyclase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-111)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13444.56 Da
• Isoelectric Point: 5.1881

>T69325 WP_003405865.1 NZ_CP018300:1235577-1235951 [Mycobacterium tuberculosis]
VILVDTSVWIEHLRAADARLVELLGDDEAGCHPLVIEELALGSIKQRDVVLDLLANLYQFPVVTHDEVLRLVGRRRLWGR
GLGAVDANLLGSVALVGGARLWTRDKRLKAACAESGVALAEEVS

Nucleotide

• Length: 375 bp

>T69325 NZ_CP018300:1235577-1235951 [Mycobacterium tuberculosis]
GTGATCCTGGTCGACACTTCGGTATGGATTGAGCACCTGCGCGCCGCCGACGCGCGACTCGTCGAGCTGCTGGGCGATGA
CGAGGCCGGTTGCCATCCGCTCGTCATCGAGGAGCTGGCGCTTGGCTCGATCAAGCAGCGAGACGTTGTTCTCGATCTGT
TGGCCAACCTCTACCAGTTTCCGGTGGTGACCCACGACGAAGTGTTGCGGCTTGTCGGTCGGCGGCGGTTGTGGGGTCGG
GGACTCGGTGCCGTCGATGCCAACCTTCTTGGTTCGGTGGCTCTGGTTGGCGGCGCGCGACTATGGACGCGGGACAAGCG
GTTGAAGGCGGCGTGCGCGGAAAGCGGTGTTGCGCTGGCTGAGGAAGTGTCCTGA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 6973.03 Da
• Isoelectric Point: 11.7327

>AT69325 WP_003405863.1 NZ_CP018300:1235383-1235580 [Mycobacterium tuberculosis]
MRTTVTVDDALLAKAAELTGVKEKSTLLREGLQTLVRVESARRLAALGGTDPQATAAPRRRTSPR

Nucleotide

• Length: 198 bp

>AT69325 NZ_CP018300:1235383-1235580 [Mycobacterium tuberculosis]
ATGAGGACGACGGTGACCGTTGACGACGCCTTGTTAGCCAAAGCGGCCGAATTGACTGGGGTGAAAGAGAAGTCGACGCT
CCTGCGCGAGGGGTTGCAGACACTGGTCCGGGTGGAGAGCGCCCGGCGGTTGGCGGCTCTCGGCGGCACCGACCCGCAAG
CTACCGCGGCGCCGAGACGCCGGACGTCGCCCCGGTGA

Structures

Toxin

• AlphaFold DB: G0TH08

Antitoxin

• AlphaFold DB: G0TH07