Detailed information of TA system TA069301

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA(toxin)
• Location: 5989..6566
• Replicon: plasmid pXH901
• Accession: NZ_CP018261
• Organism: Acinetobacter haemolyticus strain XH900

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: BSR56_RS15975
• Protein ID: WP_004897309.1
• Coordinates: 6318..6566 (-)
• Length: 83 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: BSR56_RS15970
• Protein ID: WP_004897307.1
• Coordinates: 5989..6321 (-)
• Length: 111 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BSR56_RS15940	1800..2066	+	267	Protein_2	transposase	-
BSR56_RS15945	2278..3231	+	954	Protein_3	IS4 family transposase	-
BSR56_RS15950	3306..3689	+	384	WP_001055589.1	IS66 family insertion sequence hypothetical protein	-
BSR56_RS15955	3724..4020	+	297	WP_079398519.1	IS66 family insertion sequence element accessory protein TnpB	-
BSR56_RS15960	4095..5678	+	1584	WP_044099894.1	IS66-like element ISAba25 family transposase	-
BSR56_RS15965	5707..5925	+	219	Protein_7	IS4 family transposase	-
BSR56_RS15970	5989..6321	-	333	WP_004897307.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
BSR56_RS15975	6318..6566	-	249	WP_004897309.1	type II toxin-antitoxin system HicA family toxin	Toxin
BSR56_RS15980	6722..7003	+	282	WP_004897311.1	hypothetical protein	-
BSR56_RS15985	7342..7566	-	225	WP_004758944.1	hypothetical protein	-
BSR56_RS15990	7721..7966	+	246	WP_004282220.1	hypothetical protein	-
BSR56_RS15995	8081..8236	+	156	Protein_13	IS5/IS1182 family transposase	-
BSR56_RS16000	8316..8933	+	618	WP_004758942.1	AAA family ATPase	-
BSR56_RS16005	8933..9931	+	999	WP_004758941.1	ParB/RepB/Spo0J family partition protein	-
BSR56_RS16010	10053..11363	-	1311	WP_004758940.1	replication initiation protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..54698	54698

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (20-78)

Antitoxin

• Uncharacterised protein family HicB (54-101)

Sequences

Toxin

Protein

• Length: 83 a.a.
• Molecular weight: 9501.14 Da
• Isoelectric Point: 10.3185

>T69301 WP_004897309.1 NZ_CP018261:c6566-6318 [Acinetobacter haemolyticus]
MGKTEKLLEKLGNSKNTFPYKDLVVLLSQLGYEKFEMAGSRVRFFNEKTEHMILLHRPHPENEIKGGALKAVKQALKQER
FL

Nucleotide

• Length: 249 bp

>T69301 NZ_CP018261:c6566-6318 [Acinetobacter haemolyticus]
ATGGGTAAAACGGAAAAGTTATTGGAAAAACTTGGAAATTCAAAAAATACATTTCCATATAAAGATTTAGTCGTTTTACT
AAGCCAACTCGGTTATGAAAAGTTTGAAATGGCAGGCTCTCGTGTTCGTTTTTTTAATGAAAAAACTGAACATATGATTT
TACTGCATAGACCACACCCTGAGAATGAAATCAAAGGCGGTGCATTGAAAGCTGTAAAACAGGCACTTAAGCAGGAGAGA
TTTTTATGA

Antitoxin

Protein

• Length: 111 a.a.
• Molecular weight: 12408.18 Da
• Isoelectric Point: 4.8838

>AT69301 WP_004897307.1 NZ_CP018261:c6321-5989 [Acinetobacter haemolyticus]
MSYLKYKGYLGTIEPDLETGELFGKVAFIRDLITYEAETLKALEQAFQESVDGYLESCIELNKKPDQPFKGTFNVRISPD
LHRKAVLASSNSLNNFVSDAIQEKLMRLGV

Nucleotide

• Length: 333 bp

>AT69301 NZ_CP018261:c6321-5989 [Acinetobacter haemolyticus]
ATGAGCTATCTAAAATATAAAGGTTATCTTGGAACAATTGAGCCTGATTTAGAAACAGGTGAGTTGTTTGGAAAAGTTGC
TTTTATACGTGATTTAATCACTTATGAAGCCGAAACATTAAAAGCATTAGAACAAGCTTTTCAAGAATCTGTAGATGGCT
ATTTAGAATCTTGTATCGAACTAAATAAAAAACCAGATCAGCCTTTTAAAGGTACGTTTAATGTACGGATTAGCCCTGAT
CTACACCGTAAAGCTGTTTTAGCATCAAGTAATTCCTTGAATAACTTTGTAAGCGATGCAATTCAAGAAAAGCTAATGCG
TCTAGGTGTTTAA