Detailed information of TA system TA069294

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 3222856..3223441
• Replicon: chromosome
• Accession: NZ_CP018259
• Organism: Acinetobacter bereziniae strain XH901

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: N8YNI6
• Locus tag: BSR55_RS15210
• Protein ID: WP_004829342.1
• Coordinates: 3222856..3223143 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: -
• Locus tag: BSR55_RS15215
• Protein ID: WP_035300040.1
• Coordinates: 3223130..3223441 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BSR55_RS15180	3218560..3219231	+	672	WP_100885672.1	7-cyano-7-deazaguanine synthase QueC	-
BSR55_RS15185	3219245..3219655	+	411	WP_004829333.1	hypothetical protein	-
BSR55_RS15190	3219719..3220420	+	702	WP_004829335.1	diphthine--ammonia ligase	-
BSR55_RS15195	3220424..3220885	+	462	WP_004829336.1	peroxiredoxin	-
BSR55_RS15200	3221037..3221636	-	600	WP_100885673.1	LysE/ArgO family amino acid transporter	-
BSR55_RS15205	3221814..3222704	+	891	WP_100885674.1	LysR family transcriptional regulator ArgP	-
BSR55_RS15210	3222856..3223143	+	288	WP_004829342.1	BrnT family toxin	Toxin
BSR55_RS15215	3223130..3223441	+	312	WP_035300040.1	BrnA antitoxin family protein	Antitoxin
BSR55_RS15220	3223515..3223826	+	312	WP_004829346.1	hypothetical protein	-
BSR55_RS15225	3224200..3224400	+	201	Protein_2940	hypothetical protein	-
BSR55_RS15230	3224633..3225238	-	606	WP_004829348.1	glutathione transferase GstA	-
BSR55_RS15235	3225336..3226253	+	918	WP_100885675.1	LysR family transcriptional regulator	-
BSR55_RS15240	3226304..3226870	-	567	WP_004829351.1	YceI family protein	-
BSR55_RS15245	3227210..3227776	-	567	WP_004829353.1	nicotinate-nicotinamide nucleotide adenylyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (41-96)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11281.67 Da
• Isoelectric Point: 4.6528

>T69294 WP_004829342.1 NZ_CP018259:3222856-3223143 [Acinetobacter bereziniae]
MELYFEWDEEKNQKNQKKHDVSFETASLIFEDPLRISIQDRHTDGEERWQSIGMVKGVLMLLVAHTIFDEDDCEIIRIIS
ARQVTKAERDKYEHG

Nucleotide

• Length: 288 bp

>T69294 NZ_CP018259:3222856-3223143 [Acinetobacter bereziniae]
ATGGAACTGTATTTCGAATGGGATGAGGAAAAGAACCAAAAGAATCAGAAAAAACATGATGTCTCTTTTGAAACAGCAAG
CCTCATTTTTGAAGATCCATTACGGATATCAATCCAAGATAGACATACTGATGGCGAAGAACGTTGGCAATCCATTGGTA
TGGTTAAAGGCGTACTAATGCTTTTAGTTGCTCACACCATCTTTGATGAAGATGACTGTGAAATCATACGAATCATTAGT
GCAAGACAAGTAACCAAAGCAGAGCGGGATAAATATGAGCATGGTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 12141.78 Da
• Isoelectric Point: 8.4944

>AT69294 WP_035300040.1 NZ_CP018259:3223130-3223441 [Acinetobacter bereziniae]
MSMVRYTRKELNDNFSDKQDAEIKRLLEKGSTPDEQLDLSDIPEITEWSHAVRHGQFYRPVKQQTSVRLDADVLAWLKEQ
GKGYQTRINKILREAMLKDLKNH

Nucleotide

• Length: 312 bp

>AT69294 NZ_CP018259:3223130-3223441 [Acinetobacter bereziniae]
ATGAGCATGGTTAGATATACACGCAAAGAACTGAATGATAATTTCAGTGACAAGCAAGATGCTGAAATTAAACGCTTGCT
TGAGAAAGGATCAACTCCTGATGAGCAATTAGACTTATCGGATATTCCTGAAATTACTGAGTGGAGTCATGCTGTTCGTC
ATGGGCAATTCTATCGCCCAGTGAAGCAACAGACTTCTGTTCGCCTTGATGCTGATGTACTTGCATGGTTAAAAGAACAA
GGTAAAGGCTATCAGACACGCATAAATAAAATTCTGCGTGAAGCTATGTTAAAAGATTTAAAAAATCATTAA

Structures

Toxin

• AlphaFold DB: N8YNI6