Detailed information of TA system TA069281

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/PRK09812-ChpS
• Location: 5320879..5321474
• Replicon: chromosome
• Accession: NZ_CP018252
• Organism: Escherichia coli strain 9000

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: U9Y4M4
• Locus tag: BFL16_RS29365
• Protein ID: WP_000239579.1
• Coordinates: 5321124..5321474 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: Q8XCF3
• Locus tag: BFL16_RS29360
• Protein ID: WP_001223210.1
• Coordinates: 5320879..5321130 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BFL16_RS29350	5316544..5320323	+	3780	WP_000060930.1	autotransporter assembly complex protein TamB	-
BFL16_RS29355	5320326..5320667	+	342	WP_001219160.1	gamma-glutamylcyclotransferase	-
BFL16_RS29360	5320879..5321130	+	252	WP_001223210.1	type II toxin-antitoxin system antitoxin ChpS	Antitoxin
BFL16_RS29365	5321124..5321474	+	351	WP_000239579.1	endoribonuclease toxin ChpB	Toxin
BFL16_RS29370	5321554..5322084	-	531	WP_000055075.1	inorganic diphosphatase	-
BFL16_RS29375	5322394..5323350	+	957	WP_000265942.1	galactofuranose ABC transporter substrate-binding protein YtfQ	-
BFL16_RS29380	5323490..5324992	+	1503	WP_000205806.1	sugar ABC transporter ATP-binding protein	-
BFL16_RS29385	5325006..5326028	+	1023	WP_001301928.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-114)

Antitoxin

• SpoVT-AbrB domain (6-49)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12511.45 Da
• Isoelectric Point: 5.6219

>T69281 WP_000239579.1 NZ_CP018252:5321124-5321474 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAADEVVEEALLRLQAVVE

Nucleotide

• Length: 351 bp

>T69281 NZ_CP018252:5321124-5321474 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GTGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTT
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9299.64 Da
• Isoelectric Point: 4.4201

>AT69281 WP_001223210.1 NZ_CP018252:5320879-5321130 [Escherichia coli]
MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEVQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Nucleotide

• Length: 252 bp

>AT69281 NZ_CP018252:5320879-5321130 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGTGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Structures

Toxin

• AlphaFold DB: A0A0E0Y8A8