Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 4398284..4398915 Replicon chromosome
Accession NZ_CP018252
Organism Escherichia coli strain 9000

Toxin (Protein)


Gene name hicA Uniprot ID Q8X5S6
Locus tag BFL16_RS24585 Protein ID WP_001259386.1
Coordinates 4398640..4398915 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag BFL16_RS24580 Protein ID WP_000593555.1
Coordinates 4398284..4398643 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BFL16_RS24550 4393396..4393869 + 474 WP_001161553.1 PTS sugar transporter subunit IIA -
BFL16_RS24555 4393866..4394147 + 282 WP_000084025.1 PTS sugar transporter subunit IIB -
BFL16_RS24560 4394224..4395582 + 1359 WP_001302220.1 PTS galactitol transporter subunit IIC -
BFL16_RS24565 4395575..4397083 + 1509 WP_001301773.1 carbohydrate kinase -
BFL16_RS24570 4397073..4397342 + 270 WP_000933060.1 HPr family phosphocarrier protein -
BFL16_RS24575 4397374..4398234 + 861 WP_001131788.1 class II aldolase -
BFL16_RS24580 4398284..4398643 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
BFL16_RS24585 4398640..4398915 - 276 WP_001259386.1 type II toxin-antitoxin system HicA family toxin Toxin
BFL16_RS24590 4398988..4400112 - 1125 WP_001301659.1 ABC transporter permease -
BFL16_RS24595 4400112..4402847 - 2736 WP_000149107.1 ribosome-associated ATPase/putative transporter RbbA -
BFL16_RS24600 4402844..4403911 - 1068 WP_000361458.1 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10165.92 Da        Isoelectric Point: 10.7076

>T69277 WP_001259386.1 NZ_CP018252:c4398915-4398640 [Escherichia coli]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKLILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T69277 NZ_CP018252:c4398915-4398640 [Escherichia coli]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGC
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT69277 WP_000593555.1 NZ_CP018252:c4398643-4398284 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT69277 NZ_CP018252:c4398643-4398284 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1I9WXY7

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References