Detailed information of TA system TA069235

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/DUF1778(antitoxin)
• Location: 4285354..4286154
• Replicon: chromosome
• Accession: NZ_CP018250
• Organism: Escherichia coli strain 10671

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: A0A891SNC1
• Locus tag: BFL17_RS23650
• Protein ID: WP_000342448.1
• Coordinates: 4285354..4285881 (-)
• Length: 176 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: -
• Locus tag: BFL17_RS23655
• Protein ID: WP_072608505.1
• Coordinates: 4285888..4286154 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BFL17_RS23620	4280429..4281196	-	768	WP_000082101.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-
BFL17_RS23625	4281193..4282470	-	1278	WP_000803797.1	branched chain amino acid ABC transporter permease LivM	-
BFL17_RS23630	4282467..4283393	-	927	WP_001295111.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
BFL17_RS23635	4283441..4284550	-	1110	WP_001301528.1	high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK	-
BFL17_RS23645	4284974..4285357	+	384	WP_000778769.1	aspartate 1-decarboxylase autocleavage activator PanM	-
BFL17_RS23650	4285354..4285881	-	528	WP_000342448.1	GNAT family N-acetyltransferase	Toxin
BFL17_RS23655	4285888..4286154	-	267	WP_072608505.1	DUF1778 domain-containing protein	Antitoxin
BFL17_RS23660	4286304..4287407	-	1104	WP_001021996.1	branched chain amino acid ABC transporter substrate-binding protein LivJ	-
BFL17_RS23665	4287678..4288532	-	855	WP_000130217.1	RNA polymerase sigma factor RpoH	-
BFL17_RS23670	4288777..4289835	-	1059	WP_001042003.1	permease-like cell division protein FtsX	-
BFL17_RS23675	4289828..4290496	-	669	WP_000617723.1	cell division ATP-binding protein FtsE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (88-150)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 176 a.a.
• Molecular weight: 19692.68 Da
• Isoelectric Point: 7.3232

>T69235 WP_000342448.1 NZ_CP018250:c4285881-4285354 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNEKAHTFYKSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Nucleotide

• Length: 528 bp

>T69235 NZ_CP018250:c4285881-4285354 [Escherichia coli]
ATGGATGATCTGACGATAGAGATTCTGACCGATGATGCAGATTATGATCTACAGCGATTCGACTGCGGCGAGGAAGCGTT
AAATCTCTTTCTGACGACACATCTCGTTCGTCAACATCGCAACAAAATTCTGCGAGCGTATATCCTTTGTCGCAACACTC
CAGAACGTCAGGTGCTGGGATATTACACATTATGCGGCAGTTGTTTTGAACGAGCCGCATTGCCCTCGAAATCGAAACAG
AAAAAAATTCCCTACAAAAATATTCCCAGCGTTACTCTTGGGCGTCTGGCAATTGATCGTTCATTACAGGGGCAGGGATG
GGGAGCAACACTGGTTGCTCATGCCATGAAAGTCGTCTGGTCAGCCTCTTTAGCGGTAGGTATTCACGGTCTTTTTGTCG
AGGCGCTGAATGAAAAAGCCCATACGTTTTATAAATCGCTGGGCTTTATCCCTTTAGTCGGGGAAAACGAAAATGCGTTA
TTTTTCCCAACCAAATCCATTGAACTGCTTTTTACACAGAGCGATTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9852.28 Da
• Isoelectric Point: 10.4747

>AT69235 WP_072608505.1 NZ_CP018250:c4286154-4285888 [Escherichia coli]
MSAVKKQRIDLRLTDGDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Nucleotide

• Length: 267 bp

>AT69235 NZ_CP018250:c4286154-4285888 [Escherichia coli]
ATGTCTGCTGTTAAAAAGCAGCGTATCGATCTGCGTTTAACTGACGGTGACAAAAGTATGATTGAGGAAGCGGCAGCGAT
ATCTAATCAGTCCGTCAGCCAATTTATGTTGAACAGCGCTTCGCAGCGGGCTGCGGAAGTGATTGAACAGCATCGGCGGG
TGATCCTCAATGAGGAATCCTGGACGCGGGTGATGGATGCGCTGAGTAATCCACCGTCACCAGGTGAAAAGCTAAAACGT
GCGGCAAAACGTCTTCAGGGAATGTAA

Structures

Toxin

• PDB: 6GTS