Detailed information of TA system TA069215

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1128581..1129376
• Replicon: chromosome
• Accession: NZ_CP018250
• Organism: Escherichia coli strain 10671

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: B7UP43
• Locus tag: BFL17_RS05895
• Protein ID: WP_000854914.1
• Coordinates: 1129002..1129376 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A0B0VS41
• Locus tag: BFL17_RS05890
• Protein ID: WP_001280954.1
• Coordinates: 1128581..1128955 (+)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BFL17_RS05845	1123936..1124841	+	906	WP_000203541.1	chemotaxis protein	-
BFL17_RS05850	1124838..1125908	+	1071	WP_000102669.1	patatin-like phospholipase family protein	-
BFL17_RS05865	1126248..1127066	+	819	WP_001234682.1	DUF945 domain-containing protein	-
BFL17_RS05870	1127157..1127642	+	486	WP_000214398.1	antirestriction protein	-
BFL17_RS05875	1127658..1128134	+	477	WP_001186738.1	RadC family protein	-
BFL17_RS05880	1128197..1128418	+	222	WP_001220314.1	DUF987 domain-containing protein	-
BFL17_RS05890	1128581..1128955	+	375	WP_001280954.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
BFL17_RS05895	1129002..1129376	+	375	WP_000854914.1	TA system toxin CbtA family protein	Toxin
BFL17_RS05900	1129373..1129864	+	492	WP_000976853.1	hypothetical protein	-
BFL17_RS05905	1129876..1130073	+	198	WP_000839282.1	DUF957 domain-containing protein	-
BFL17_RS05910	1130158..1131000	+	843	WP_001280481.1	DUF4942 domain-containing protein	-
BFL17_RS05920	1131491..1131709	-	219	WP_001040187.1	translation initiation factor IF-1	-
BFL17_RS05930	1131994..1132698	-	705	WP_001241680.1	leucyl/phenylalanyl-tRNA--protein transferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14117.17 Da
• Isoelectric Point: 7.7761

>T69215 WP_000854914.1 NZ_CP018250:1129002-1129376 [Escherichia coli]
MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T69215 NZ_CP018250:1129002-1129376 [Escherichia coli]
ATGAAAACATTATCCGACACACATGTACGGGAGGTATCTCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAT
CCGACTGCTGGACCAGCATTATGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTTAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACCTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 13718.51 Da
• Isoelectric Point: 6.6249

>AT69215 WP_001280954.1 NZ_CP018250:1128581-1128955 [Escherichia coli]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRYQHTVTLNAKGLTCEADTLGSCGYVYLAVYPTPAPATTS

Nucleotide

• Length: 375 bp

>AT69215 NZ_CP018250:1128581-1128955 [Escherichia coli]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGATGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGCGCTCGTCTGGTGCAAGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGATATCAGCATACCGTCACGCTGAATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCAGCACCCGCAACCACCTCATAA

Structures

Toxin

• AlphaFold DB: A0A7U9LXR5

Antitoxin

• AlphaFold DB: A0A0B0VS41